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. 1983 Jul 11;11(13):4583–4600. doi: 10.1093/nar/11.13.4583

Conformational analysis of oligoarabinonucleotides. An NMR and CD study.

J Doornbos, J L Barascut, H Lazrek, J L Imbach, J van Westrenen, G M Visser, J H van Boom, C Altona
PMCID: PMC326066  PMID: 6866774

Abstract

A 500 and 300 MHz proton NMR study of the series of oligoarabinonucleotides 5'aAMP, 3'aAMP, aA-aA, (aA-)2aA and (aA-)3aA is presented. In addition, circular dichroism is used to study the stacking behaviour of aA-aA. The complete 1H-NMR spectral assignment of the compounds (except the tetramer) is given. Proton-proton and proton-phosphorus coupling constants, obtained by computer simulation of the high-field region of the spectra, yield information on the conformation of the arabinose rings (N- or S-type) and on the intramolecular stacking properties of the dimer and the trimer. The monomers 5'aAMP and 3'aAMP exhibit a preference for N- and S-type sugar conformation, respectively. It is shown that the dimer aA-aA at low temperature prefers a mixed stacked state of the type aA(S)-aA(N). In the trimer the aA(2)-aA(3) fragment exhibits a conformation similar to that found in the dimer, whereas the aA(1) residue prefers to adopt S-type sugar and has some tendency to stack upon residue aA(2).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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