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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Methods. 2011 Sep 8;55(4):370–378. doi: 10.1016/j.ymeth.2011.08.019

Table 1.

C43(DE3) amino-acid auxotrophs constructed with the λ-Red recombination system

Strain Genes deleted Amino acids required for growth
ML2 ilvE Ile, Leua
ML3 hisG His
ML4 avtA Val
ML5 metA Met
ML6 ilvE avtA Ile, Val, Leua
ML8 argH Arg
ML12 ilvE avtA aspC Ile, Val, Leua, Tyrb
ML14 tyrA Tyr
ML17 glnA Gln
ML21 hisG tyrA His, Tyr
ML22 ilvE avtA aspC hisG Ile, Val, His, Leua, Tyrb
ML26 ilvE avtA aspC hisG argH Ile, Val, His, Arg, Leua, Tyrb
ML30 ilvE avtA aspC hisG argH glnA Ile, Val, His, Arg, Gln, Leua, Tyrb
ML31 ilvE avtA aspC hisG argH metA Ile, Val, His, Arg, Met, Leua, Tyrb
ML32 argH glnA Arg, Gln
ML34 argH glnA hisG Arg, Gln, His
ML36 ilvE avtA aspC hisG metA Ile, Val, His, Met, Leua, Tyrb
ML40 ilvE avtA aspC hisG argH metA lysA Ile, Val, His, Arg, Met, Lys, Leua, Tyrb
ML41 ilvE avtA aspC hisG argH metA lysA thrC Ile, Val, His, Arg, Met, Lys, Thr, Leua, Tyrb
C43_cysE cysE Cys
a

In the presence of 0.4–1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium.

b

Addition of 0.4–1 mM Tyr represses tyrB, allowing these strains to be used for isotope labeling of Tyr.