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. 1982 Jan 22;10(2):539–546. doi: 10.1093/nar/10.2.539

The genome of Plasmodium falciparum. I: DNA base composition.

Y Pollack, A L Katzen, D T Spira, J Golenser
PMCID: PMC326156  PMID: 6278419

Abstract

Some structural properties of the DNA of Plasmodium falciparum were studied thoroughly using several techniques. Its G+C content was found to be extremely low (17-19%), the lowest reported for a living organism. The DNA seems to be composed only of the four major bases as no methylated bases were detected. This DNA had a Tm value of 62.5 degrees C and its denaturation profile showed no marked intramolecular heterogeneity.

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Selected References

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  1. De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol. 1970 Mar;101(3):738–754. doi: 10.1128/jb.101.3.738-754.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gruenbaum Y., Stein R., Cedar H., Razin A. Methylation of CpG sequences in eukaryotic DNA. FEBS Lett. 1981 Feb 9;124(1):67–71. doi: 10.1016/0014-5793(81)80055-5. [DOI] [PubMed] [Google Scholar]
  3. Gutteridge W. E., Trigg P. I., Williamson D. H. Properties of DNA from some malarial parasites. Parasitology. 1971 Apr;62(2):209–219. doi: 10.1017/s0031182000071456. [DOI] [PubMed] [Google Scholar]
  4. Hessling J. J., Miller S. E., Levy N. L. A direct comparison of procedures for the detection of mycoplasma in tissue culture. J Immunol Methods. 1980;38(3-4):315–324. doi: 10.1016/0022-1759(80)90280-x. [DOI] [PubMed] [Google Scholar]
  5. Homewood C. A., Neame K. D. A comparison of methods used for the removal of white cells from malaria-infected blood. Ann Trop Med Parasitol. 1976 Jun;70(2):249–251. doi: 10.1080/00034983.1976.11687119. [DOI] [PubMed] [Google Scholar]
  6. MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
  7. Owen R. J., Hill L. R., Lapage S. P. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers. 1969;7(4):503–516. doi: 10.1002/bip.1969.360070408. [DOI] [PubMed] [Google Scholar]
  8. Razin A., Friedman J. DNA methylation and its possible biological roles. Prog Nucleic Acid Res Mol Biol. 1981;25:33–52. doi: 10.1016/s0079-6603(08)60482-1. [DOI] [PubMed] [Google Scholar]
  9. Razin A., Razin S. Methylated bases in mycoplasmal DNA. Nucleic Acids Res. 1980 Mar 25;8(6):1383–1390. doi: 10.1093/nar/8.6.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Razin A., Riggs A. D. DNA methylation and gene function. Science. 1980 Nov 7;210(4470):604–610. doi: 10.1126/science.6254144. [DOI] [PubMed] [Google Scholar]
  11. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  12. SINSHEIMER R. L. The action of pancreatic deoxyribonuclease. II. Isomeric dinucleotides. J Biol Chem. 1955 Aug;215(2):579–583. [PubMed] [Google Scholar]
  13. Singer J., Stellwagen R. H., Roberts-Ems J., Riggs A. D. 5-Methylcytosine content of rat hepatoma DNA substituted with bromodeoxyuridine. J Biol Chem. 1977 Aug 10;252(15):5509–5513. [PubMed] [Google Scholar]
  14. Walsh C. J., Sherman I. W. Isolation, characterization and synthesis of DNA from a malaria parasite. J Protozool. 1968 Aug;15(3):503–508. doi: 10.1111/j.1550-7408.1968.tb02163.x. [DOI] [PubMed] [Google Scholar]

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