Abstract
Some structural properties of the DNA of Plasmodium falciparum were studied thoroughly using several techniques. Its G+C content was found to be extremely low (17-19%), the lowest reported for a living organism. The DNA seems to be composed only of the four major bases as no methylated bases were detected. This DNA had a Tm value of 62.5 degrees C and its denaturation profile showed no marked intramolecular heterogeneity.
Full text
PDF







Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- De Ley J. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol. 1970 Mar;101(3):738–754. doi: 10.1128/jb.101.3.738-754.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gruenbaum Y., Stein R., Cedar H., Razin A. Methylation of CpG sequences in eukaryotic DNA. FEBS Lett. 1981 Feb 9;124(1):67–71. doi: 10.1016/0014-5793(81)80055-5. [DOI] [PubMed] [Google Scholar]
- Gutteridge W. E., Trigg P. I., Williamson D. H. Properties of DNA from some malarial parasites. Parasitology. 1971 Apr;62(2):209–219. doi: 10.1017/s0031182000071456. [DOI] [PubMed] [Google Scholar]
- Hessling J. J., Miller S. E., Levy N. L. A direct comparison of procedures for the detection of mycoplasma in tissue culture. J Immunol Methods. 1980;38(3-4):315–324. doi: 10.1016/0022-1759(80)90280-x. [DOI] [PubMed] [Google Scholar]
- Homewood C. A., Neame K. D. A comparison of methods used for the removal of white cells from malaria-infected blood. Ann Trop Med Parasitol. 1976 Jun;70(2):249–251. doi: 10.1080/00034983.1976.11687119. [DOI] [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
- Owen R. J., Hill L. R., Lapage S. P. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers. 1969;7(4):503–516. doi: 10.1002/bip.1969.360070408. [DOI] [PubMed] [Google Scholar]
- Razin A., Friedman J. DNA methylation and its possible biological roles. Prog Nucleic Acid Res Mol Biol. 1981;25:33–52. doi: 10.1016/s0079-6603(08)60482-1. [DOI] [PubMed] [Google Scholar]
- Razin A., Razin S. Methylated bases in mycoplasmal DNA. Nucleic Acids Res. 1980 Mar 25;8(6):1383–1390. doi: 10.1093/nar/8.6.1383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin A., Riggs A. D. DNA methylation and gene function. Science. 1980 Nov 7;210(4470):604–610. doi: 10.1126/science.6254144. [DOI] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- SINSHEIMER R. L. The action of pancreatic deoxyribonuclease. II. Isomeric dinucleotides. J Biol Chem. 1955 Aug;215(2):579–583. [PubMed] [Google Scholar]
- Singer J., Stellwagen R. H., Roberts-Ems J., Riggs A. D. 5-Methylcytosine content of rat hepatoma DNA substituted with bromodeoxyuridine. J Biol Chem. 1977 Aug 10;252(15):5509–5513. [PubMed] [Google Scholar]
- Walsh C. J., Sherman I. W. Isolation, characterization and synthesis of DNA from a malaria parasite. J Protozool. 1968 Aug;15(3):503–508. doi: 10.1111/j.1550-7408.1968.tb02163.x. [DOI] [PubMed] [Google Scholar]