Table 2.
Clinical information and summary for Tax-specific CD8+ T cells in 14ACs
| ID | Age | Sex | WBC (/μl) |
CD4 (%)1 | CD8 (%)1 | HLA | Tetramer (%)2 | Functions and phenotype of Tax-specific CD8+ T-cells3 |
Ably (%)7 | PVL8 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IFN-γ+ (%)4 |
CFSElow (%)5 |
PD-1+ (%)6 |
||||||||||
| #217 | 70s | F | 6800 | ND9 | 5.72 | A24 | 1.94 | 27.7 | 78.9 | 78.7 | 0 | 14 |
| #236 | 30 s | F | 6500 | ND | 11.9 | A24 | 2.54 | 31.1 | 0 | 54.1 | 0 | 22 |
| #238 | 60 s | F | 5700 | ND | 12.7 | A11 | 1.29 | 36.4 | 100 | 0 | 0 | 2 |
| #243 | 50 s | F | 4100 | ND | 24.6 | A2/24 | 0.39/3.67 | 11.3 | 27.6 | 93.8 | 0 | 3 |
| #245 | 40 s | F | 5000 | ND | 22.6 | A2 | 0.73 | 62.5 | 75 | ND | 1 | 58 |
| #251 | 60 s | M | 4800 | ND | 11.9 | A2/11 | 0.70/8.23 | 35.8 | 84.4 | 36.7 | 0 | 2 |
| #279 | 40 s | M | 6200 | 34.1 | 11.6 | A2/24 | 4.70/0.18 | 12.9 | 30.8 | 70.2 | 1 | 48 |
| #287 | 70 s | M | 4800 | 72.5 | 10.0 | A2/24 | 1.17/0.23 | 11.1 | 0 | 55.6 | 2 | 81 |
| #309 | 60 s | F | 4600 | 37.5 | 24.8 | A11/24 | 6.88/4.26 | 51.7 | 76.2 | 85.3 | 1.5 | 29 |
| #311 | 60 s | F | 3200 | 30.6 | 14.8 | A2/24 | 1.02/1.94 | 51.3 | ND | ND | 0 | 6 |
| #312 | 50 s | F | 2700 | 27.3 | 36.4 | A24 | 2.03 | 77.8 | ND | ND | ND | UN10 |
| #313 | 60 s | M | 7300 | 25.4 | 31.0 | A24 | 1.11 | 55.7 | 60 | 90.6 | ND | 4 |
| #315 | 50 s | F | 7500 | 26.5 | 7.9 | A2/24 | 6.88/0 | 24.5 | 84.7 | 20 | 0.6 | 17 |
| #360 | 50 s | M | 6200 | 37.7 | 29.9 | A2 | 2.6 | 63.1 | 68.4 | 10.2 | 0 | UN |
1The number indicates percentage of CD4+ or CD8+ T cells in lymphocytes.
2The number indicates percentages of tetramer+ cells in CD8+ T-cells. Two numbers divided by a slash represent those detected by two different tetramers corresponding to two HLA alleles shown in the HLA column.
3In case of a sample carrying two of three HLA-A alleles (A2, A11, or A24), Tax-specific CTLs predominantly detected by a tetramer were used. The number represents percentage of indicated cells in the tetramer-binding CD8+ T cells.
4Evaluated by intracellular IFN-γ staining following 6 hours stimulation with corresponding Tax peptide.
5Evaluated by CFSE intensities in labeled PBMC after 6 days incubation with corresponding Tax peptide stimulation.
6The number represents percentage of indicated PD-1+Tax-specific CD8+ T cells without culture.
7Ably; abnormal lymphocytes
8PVL; proviral load. The number represents copy number per 1000 PBMCs.
9ND; not determined
10UN; undetectable