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. 1982 Feb 11;10(3):855–869. doi: 10.1093/nar/10.3.855

Overlapping genes at the DNA primase locus of the large plasmid ColI.

G J Boulnois, B M Wilkins, E Lanka
PMCID: PMC326207  PMID: 6278439

Abstract

The sog gene of the large plasmid ColIdrd-1 has previously been shown to encode a DNA primase and a smaller antigenically related polypeptide. Genesis of these two products has been examined using Sog+ recombinant plasmids. Effects of amber mutations, isolated after in vitro mutagenesis, and deletions into or within sog suggest that the smaller polypeptide is a separate translation product which is encoded by DNA specifying the C-terminal region of the larger protein. Under control of the lac promotor, synthesis of both polypeptides is reduced when transcription is repressed. These findings imply that transcription of sog yields a single transcript which is translated from two initiation sites.

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Selected References

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