Abstract
A cloned restriction fragment of maize chloroplast DNA (Bam H1 fragment 5) is shown to contain an open reading frame which encodes a basic protein of 201 amino acid residues with 40-50% sequence homology to E. coli ribosomal protein S4. Based on the experimentally determined sequence homology between the highly conserved bacterial ribosomal protein L12 and its chloroplast homologue (Bartsch M., Kimura, M. and Subramanian, A.R. (1982) Proc. Natl. Acad. Sci. USA 79, 6871), we conclude that this reading frame represents the maize chloroplast S4 gene. The nucleotide sequence of a 1100 base pair DNA segment containing the putative gene is presented.
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- Bartsch M., Kimura M., Subramanian A. R. Purification, primary structure, and homology relationships of a chloroplast ribosomal protein. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6871–6875. doi: 10.1073/pnas.79.22.6871. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coen D. M., Bedbrook J. R., Bogorad L., Rich A. Maize chloroplast DNA fragment encoding the large subunit of ribulosebisphosphate carboxylase. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5487–5491. doi: 10.1073/pnas.74.12.5487. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davidson J. N., Hanson M. R., Bogorad L. Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics. 1978 Jun;89(2):281–297. doi: 10.1093/genetics/89.2.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krebbers E. T., Larrinua I. M., McIntosh L., Bogorad L. The maize chloroplast genes for the beta and epsilon subunits of the photosynthetic coupling factor CF1 are fused. Nucleic Acids Res. 1982 Aug 25;10(16):4985–5002. doi: 10.1093/nar/10.16.4985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Mizushima S., Nomura M. Assembly mapping of 30S ribosomal proteins from E. coli. Nature. 1970 Jun 27;226(5252):1214–1214. doi: 10.1038/2261214a0. [DOI] [PubMed] [Google Scholar]
- Schiltz E., Reinbolt J. Determination of the complete amino-acid sequence of protein S4 from Escherichia coli ribosomes. Eur J Biochem. 1975 Aug 15;56(2):467–481. doi: 10.1111/j.1432-1033.1975.tb02253.x. [DOI] [PubMed] [Google Scholar]
- Schnier J., Kimura M., Foulaki K., Subramanian A. R., Isono K., Wittmann-Liebold B. Primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1008–1011. doi: 10.1073/pnas.79.4.1008. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwarz Z., Jolly S. O., Steinmetz A. A., Bogorad L. Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423–3427. doi: 10.1073/pnas.78.6.3423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinmetz A. A., Krebbers E. T., Schwarz Z., Gubbins E. J., Bogorad L. Nucleotide sequences of five maize chloroplast transfer RNA genes and their flanking regions. J Biol Chem. 1983 May 10;258(9):5503–5511. [PubMed] [Google Scholar]
- Sugita M., Sugiura M. A putative gene of tobacco chloroplast coding for ribosomal protein similar to E. coli ribosomal protein S19. Nucleic Acids Res. 1983 Mar 25;11(6):1913–1918. doi: 10.1093/nar/11.6.1913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wittmann H. G. Components of bacterial ribosomes. Annu Rev Biochem. 1982;51:155–183. doi: 10.1146/annurev.bi.51.070182.001103. [DOI] [PubMed] [Google Scholar]