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. 1983 Sep 10;11(17):5795–5810. doi: 10.1093/nar/11.17.5795

In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions.

C Backendorf, J A Brandsma, T Kartasova, P van de Putte
PMCID: PMC326318  PMID: 6310514

Abstract

The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "-10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "-35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the observed basal and induced expression of the uvrA gene on a molecular level.

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Selected References

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