Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1983 Sep 10;11(17):6089–6105. doi: 10.1093/nar/11.17.6089

The tetracycline resistance determinants of RP1 and Tn1721: nucleotide sequence analysis.

S H Waters, P Rogowsky, J Grinsted, J Altenbuchner, R Schmitt
PMCID: PMC326337  PMID: 6310527

Abstract

Nucleotide sequences of the homologous tetracycline resistance (tet) determinants of plasmid RP1 and transposon Tn1721 have been determined. Two open reading frames of divergent polarity have been assigned to a regulatory gene (tetR) and a gene encoding a resistance protein (tetA). The intercistronic region contains appropriate regulatory and transcription signals. The tetR gene can code for a protein of 216 amino acids (deduced mol.wt. 23,288) and the tetA gene for a protein of 399 amino acids (deduced mol. wt. 42,205). Based on the deduced amino acid sequence, the tetA proteins of RP1/Tn1721 are 78% homologous with that of pBR322 and 45% homologous with that of Tn10. We conclude that a single tetA gene mediates resistance in each of these tet determinants.

Full text

PDF
6089

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Altenbuchner J., Choi C. L., Grinsted J., Schmitt R., Richmond M. H. The transposons Tn501(Hg) and Tn1721(Tc) are related. Genet Res. 1981 Jun;37(3):285–289. doi: 10.1017/s0016672300020280. [DOI] [PubMed] [Google Scholar]
  2. Altenbuchner J., Schmid K., Schmitt R. Tn1721-encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance. J Bacteriol. 1983 Jan;153(1):116–123. doi: 10.1128/jb.153.1.116-123.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Anderson W. F., Ohlendorf D. H., Takeda Y., Matthews B. W. Structure of the cro repressor from bacteriophage lambda and its interaction with DNA. Nature. 1981 Apr 30;290(5809):754–758. doi: 10.1038/290754a0. [DOI] [PubMed] [Google Scholar]
  4. Beck C. F., Mutzel R., Barbé J., Müller W. A multifunctional gene (tetR) controls Tn10-encoded tetracycline resistance. J Bacteriol. 1982 May;150(2):633–642. doi: 10.1128/jb.150.2.633-642.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  6. Brown N. L., Choi C. L., Grinsted J., Richmond M. H., Whitehead P. R. Nucleotide sequences at the ends of the mercury resistance transposon, Tn501. Nucleic Acids Res. 1980 May 10;8(9):1933–1945. doi: 10.1093/nar/8.9.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coleman D. C., Chopra I., Shales S. W., Howe T. G., Foster T. J. Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes. J Bacteriol. 1983 Feb;153(2):921–929. doi: 10.1128/jb.153.2.921-929.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Curiale M. S., Levy S. B. Two complementation groups mediate tetracycline resistance determined by Tn10. J Bacteriol. 1982 Jul;151(1):209–215. doi: 10.1128/jb.151.1.209-215.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ehring R., Beyreuther K., Wright J. K., Overath P. In vitro and in vivo products of E. coli lactose permease gene are identical. Nature. 1980 Feb 7;283(5747):537–540. doi: 10.1038/283537a0. [DOI] [PubMed] [Google Scholar]
  10. Grinsted J., Bennett P. M., Higginson S., Richmond M. H. Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives. Mol Gen Genet. 1978 Nov 9;166(3):313–320. doi: 10.1007/BF00267624. [DOI] [PubMed] [Google Scholar]
  11. Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hillen W., Schollmeier K. Nucleotide sequence of the Tn10 encoded tetracycline resistance gene. Nucleic Acids Res. 1983 Jan 25;11(2):525–539. doi: 10.1093/nar/11.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Jorgensen R. A., Reznikoff W. S. Organization of structural and regulatory genes that mediate tetracycline resistance in transposon Tn10. J Bacteriol. 1979 Jun;138(3):705–714. doi: 10.1128/jb.138.3.705-714.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Livneh Z. Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis. Proc Natl Acad Sci U S A. 1983 Jan;80(1):237–241. doi: 10.1073/pnas.80.1.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McMurry L., Petrucci R. E., Jr, Levy S. B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974–3977. doi: 10.1073/pnas.77.7.3974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mendez B., Tachibana C., Levy S. B. Heterogeneity of tetracycline resistance determinants. Plasmid. 1980 Mar;3(2):99–108. doi: 10.1016/0147-619x(80)90101-8. [DOI] [PubMed] [Google Scholar]
  17. Schmitt R., Bernhard E., Mattes R. Characterisation of Tn1721, a new transposon containing tetracycline resistance genes capable of amplification. Mol Gen Genet. 1979 Apr 17;172(1):53–65. doi: 10.1007/BF00276215. [DOI] [PubMed] [Google Scholar]
  18. Stalker D. M., Thomas C. M., Helinski D. R. Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2. Mol Gen Genet. 1981;181(1):8–12. doi: 10.1007/BF00338997. [DOI] [PubMed] [Google Scholar]
  19. Steitz T. A., Ohlendorf D. H., McKay D. B., Anderson W. F., Matthews B. W. Structural similarity in the DNA-binding domains of catabolite gene activator and cro repressor proteins. Proc Natl Acad Sci U S A. 1982 May;79(10):3097–3100. doi: 10.1073/pnas.79.10.3097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  21. Walz A., Pirrotta V., Ineichen K. Lambda repressor regulates the switch between PR and Prm promoters. Nature. 1976 Aug 19;262(5570):665–669. doi: 10.1038/262665a0. [DOI] [PubMed] [Google Scholar]
  22. Ward J. M., Grinsted J. Analysis of the Inc P-1 group plasmids R906 and R751 and their relationship to RP1. Plasmid. 1982 Nov;8(3):244–252. doi: 10.1016/0147-619x(82)90062-2. [DOI] [PubMed] [Google Scholar]
  23. Wiebauer K., Schraml S., Shales S. W., Schmitt R. Tetracycline resistance transposon Tn1721: recA-dependent gene amplification and expression of tetracycline resistance. J Bacteriol. 1981 Sep;147(3):851–859. doi: 10.1128/jb.147.3.851-859.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wray L. V., Jr, Jorgensen R. A., Reznikoff W. S. Identification of the tetracycline resistance promoter and repressor in transposon Tn10. J Bacteriol. 1981 Aug;147(2):297–304. doi: 10.1128/jb.147.2.297-304.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES