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Kinetic Parameters for the Reaction of HepI with LipidA Acceptor Molecules in the Presence and Absence of Detergent
| acceptor | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| A | 6.6 ± 0.8 | 29 ± 7 | 2.2 × 105 |
| A with Triton X-100 | 4.7 ± 0.3 | 9 ± 1 | 5.2 × 105 |
| B | 8 ± 1 | 46 ± 13 | 1.7 × 105 |
| B with Triton X-100 | 5.5 ± 0.4 | 16 ± 3 | 3.4 × 105 |
| C | 1.06 ± 0.05 | 0.9 ± 0.2 | 1.1 × 106 |
| C with Triton X-100 | 1.47 ± 0.08 | 0.4 ± 0.1 | 3.7 × 106 |
| D | 0.76 ± 0.03 | 0.28 ± 0.08 | 2.7 × 106 |
| D with Triton X-100 | 1.05 ± 0.07 | 0.5 ± 0.1 | 2.1 × 106 |
