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. 1983 Oct 11;11(19):6803–6819. doi: 10.1093/nar/11.19.6803

HMG 14/17 binding affinities and DNAase I sensitivities of nucleoprotein particles.

A Stein, T Townsend
PMCID: PMC326415  PMID: 6226937

Abstract

We show that ordinary (bulk) chicken erythrocyte nucleosomes are digested more rapidly by DNAase I when they are associated with high mobility group (HMG) proteins 14/17. Digestion of HMG 14/17-nucleosome complexes, under conditions where the DNA in control nucleosomes is digested to 10 to 20% acid solubility, results in a particular depletion of single-strand DNA fragments greater than 80 nucleotides in length, relative to the DNA fragments produced from control nucleosomes. Additionally, we show that staphylococcal nuclease digests of H1/H5-depleted chromatin contain an abundant subclass of nucleosomes that are not present in appreciable amounts in digests of native chromatin. These nucleosomes contain longer lengths of DNA and have lower electrophoretic mobilities than core particles. HMG 14/17 associates highly preferentially with these nucleosomes and renders them sensitive to DNAase I, similar to what has been found for active nucleosomes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albanese I., Weintraub H. Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes. Nucleic Acids Res. 1980 Jun 25;8(12):2787–2805. doi: 10.1093/nar/8.12.2787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Albright S. C., Wiseman J. M., Lange R. A., Garrard W. T. Subunit structures of different electrophoretic forms of nucleosomes. J Biol Chem. 1980 Apr 25;255(8):3673–3684. [PubMed] [Google Scholar]
  3. Anderson J. N., Vanderbilt J. N., Lawson G. M., Tsai M. J., O'Malley B. W. Chromatin structure of the ovalbumin gene family in the chicken oviduct. Biochemistry. 1983 Jan 4;22(1):21–30. doi: 10.1021/bi00270a004. [DOI] [PubMed] [Google Scholar]
  4. CROTHERS D. M., KALLENBACH N. R., ZIMM B. H. THE MELTING TRANSITION OF LOW-MOLECULAR-WEIGHT DNA: THEORY AND EXPERIMENT. J Mol Biol. 1965 Apr;11:802–820. doi: 10.1016/s0022-2836(65)80037-7. [DOI] [PubMed] [Google Scholar]
  5. Crothers D. M., Dattagupta N., Hogan M., Klevan L., Lee K. S. Transient electric dichroism studies of nucleosomal particles. Biochemistry. 1978 Oct 17;17(21):4525–4533. doi: 10.1021/bi00614a026. [DOI] [PubMed] [Google Scholar]
  6. Künzler P., Stein A. Histone H5 can increase the internucleosome spacing in dinucleosomes to nativelike values. Biochemistry. 1983 Apr 12;22(8):1783–1789. doi: 10.1021/bi00277a007. [DOI] [PubMed] [Google Scholar]
  7. Littau V. C., Burdick C. J., Allfrey V. G., Mirsky S. A. The role of histones in the maintenance of chromatin structure. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1204–1212. doi: 10.1073/pnas.54.4.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lutter L. C. Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix. J Mol Biol. 1978 Sep 15;124(2):391–420. doi: 10.1016/0022-2836(78)90306-6. [DOI] [PubMed] [Google Scholar]
  9. Mardian J. K., Paton A. E., Bunick G. J., Olins D. E. Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17. Science. 1980 Sep 26;209(4464):1534–1536. doi: 10.1126/science.7433974. [DOI] [PubMed] [Google Scholar]
  10. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McGhee J. D., Rau D. C., Felsenfeld G. The high mobility group proteins HMG 14 and 17, do not prevent the formation of chromatin higher order structure. Nucleic Acids Res. 1982 Mar 25;10(6):2007–2016. doi: 10.1093/nar/10.6.2007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mirsky A. E. The structure of chromatin. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2945–2948. doi: 10.1073/pnas.68.12.2945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sandeen G., Wood W. I., Felsenfeld G. The interaction of high mobility proteins HMG14 and 17 with nucleosomes. Nucleic Acids Res. 1980 Sep 11;8(17):3757–3778. doi: 10.1093/nar/8.17.3757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sasi R., Hüvös P. E., Fasman G. D. A conformational study of the binding of a high mobility group protein with chromatin. J Biol Chem. 1982 Oct 10;257(19):11448–11454. [PubMed] [Google Scholar]
  15. Simpson R. T. Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones. Biochemistry. 1978 Dec 12;17(25):5524–5531. doi: 10.1021/bi00618a030. [DOI] [PubMed] [Google Scholar]
  16. Simpson R. T., Whitlock J. P. Mapping DNAase l-susceptible sites in nucleosomes labeled at the 5' ends. Cell. 1976 Oct;9(2):347–353. doi: 10.1016/0092-8674(76)90124-0. [DOI] [PubMed] [Google Scholar]
  17. Sollner-Webb B., Felsenfeld G. Pancreatic DNAase cleavage sites in nuclei. Cell. 1977 Mar;10(3):537–547. doi: 10.1016/0092-8674(77)90040-x. [DOI] [PubMed] [Google Scholar]
  18. Stein A. DNA folding by histones: the kinetics of chromatin core particle reassembly and the interaction of nucleosomes with histones. J Mol Biol. 1979 May 15;130(2):103–134. doi: 10.1016/0022-2836(79)90421-2. [DOI] [PubMed] [Google Scholar]
  19. Stein A., Künzler P. Histone H5 can correctly align randomly arranged nucleosomes in a defined in vitro system. Nature. 1983 Apr 7;302(5908):548–550. doi: 10.1038/302548a0. [DOI] [PubMed] [Google Scholar]
  20. Swerdlow P. S., Varshavsky A. Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size. Nucleic Acids Res. 1983 Jan 25;11(2):387–401. doi: 10.1093/nar/11.2.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thoma F., Koller T., Klug A. Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin. J Cell Biol. 1979 Nov;83(2 Pt 1):403–427. doi: 10.1083/jcb.83.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uberbacher E. C., Mardian J. K., Rossi R. M., Olins D. E., Bunick G. J. Neutron scattering studies and modeling of high mobility group 14 core nucleosome complex. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5258–5262. doi: 10.1073/pnas.79.17.5258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weintraub H., Groudine M. Chromosomal subunits in active genes have an altered conformation. Science. 1976 Sep 3;193(4256):848–856. doi: 10.1126/science.948749. [DOI] [PubMed] [Google Scholar]
  24. Weisbrod S. T. Properties of active nucleosomes as revealed by HMG 14 and 17 chromatography. Nucleic Acids Res. 1982 Mar 25;10(6):2017–2042. doi: 10.1093/nar/10.6.2017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Weisbrod S. Active chromatin. Nature. 1982 May 27;297(5864):289–295. doi: 10.1038/297289a0. [DOI] [PubMed] [Google Scholar]
  26. Weisbrod S., Groudine M., Weintraub H. Interaction of HMG 14 and 17 with actively transcribed genes. Cell. 1980 Jan;19(1):289–301. doi: 10.1016/0092-8674(80)90410-9. [DOI] [PubMed] [Google Scholar]
  27. Weischet W. O., Allen J. R., Riedel G., Van Holde K. E. The effects of salt concentration and H-1 depletion on the digestion of calf thymus chromatin by micrococcal nuclease. Nucleic Acids Res. 1979;6(5):1843–1862. doi: 10.1093/nar/6.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]

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