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. 1983 Nov 11;11(21):7303–7316. doi: 10.1093/nar/11.21.7303

The D-JH complex is an intermediate to the complete immunoglobulin heavy-chain V-region gene.

Y Yaoita, N Matsunami, C Y Choi, H Sugiyama, T Kishimoto, T Honjo
PMCID: PMC326484  PMID: 6316256

Abstract

We have examined the organization of the immunoglobulin JH segments in three clones derived from a single Abelson murine leukemia virus-transformed cell. Cloning and nucleotide sequence analyses of the JH-containing fragments have revealed the rearrangement from the preformed D-JH complex to the complete VH-D-JH gene, which was accompanied by the expression of the intra-cytoplasmic mu chain. In one case a JH segment downstream to the preformed D-JH was used to create a new VH-D-JH gene. Upon the D-JH and VH-D-JH rearrangements the intervening D segments were deleted from the chromosome. One of the expressed VH genes suffered from a large deletion of the 3' portion (including the 95th cysteine residue) of the VH segment. We discuss the possible mechanism of the allelic exclusion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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