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. 1983 Nov 11;11(21):7487–7504. doi: 10.1093/nar/11.21.7487

Studies of the interaction of RecA protein with DNA.

D F Dombroski, D G Scraba, R D Bradley, A R Morgan
PMCID: PMC326497  PMID: 6316264

Abstract

Ethidium fluorescence assays were adapted for the rapid and sensitive detection of precA; in addition, fluorescence measurements on binding precA to linear, OC and CCC PM2 DNAs have enabled the stoichiometry of precA binding as well as the precA-induced unwinding angle of DNA to be determined. The stoichiometry of binding was independently confirmed by sedimentation analysis to be one precA molecule per 3 bp. The unwinding angle was also independently confirmed by measurements of fluorescence changes induced by the binding of precA to CCC DNA which was relaxed by topoisomerase to give a precA-induced unwinding angle of 51 degrees. Electron microscopy of OC DNA molecules which bound nonsaturating amounts of precA revealed that the length increase in DNA due to precA was approximately 55%. Finally, examination of negatively stained precA complexes with a variety of linear DNAs showed that the minor groove is the primary site of interaction for this protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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