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. 1983 Nov 25;11(22):8103–8109. doi: 10.1093/nar/11.22.8103

T4 DNA ligase can seal a nick in double-stranded DNA limited by a 5'-phosphorylated base-free deoxyribose residue.

C Goffin, W G Verly
PMCID: PMC326563  PMID: 6227866

Abstract

The 5' AP endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to AP (apurinic or apyrimidinic) sites in double-stranded DNA leaving 3'-OH and 5'-phosphate ends. These nicks are sealed by T4 DNA ligase although the 5'-phosphate end belongs to a base-free deoxyribose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clements J. E., Rogers S. G., Weiss B. A DNase for apurinic/apyrimidinic sites associated with exonuclease III of Hemophilus influenzae. J Biol Chem. 1978 May 10;253(9):2990–2999. [PubMed] [Google Scholar]
  2. Crine P., Verly W. G. Determination of single-strand breaks in DNA using neutral sucrose gradients. Anal Biochem. 1976 Oct;75(2):583–595. doi: 10.1016/0003-2697(76)90113-5. [DOI] [PubMed] [Google Scholar]
  3. César R., Verly W. G. The apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin. Eur J Biochem. 1983 Jan 1;129(3):509–517. doi: 10.1111/j.1432-1033.1983.tb07077.x. [DOI] [PubMed] [Google Scholar]
  4. Goffin C., Verly W. G. Excision of apurinic sites from DNA with enzymes isolated from rat-liver chromatin. Eur J Biochem. 1982 Oct;127(3):619–623. doi: 10.1111/j.1432-1033.1982.tb06917.x. [DOI] [PubMed] [Google Scholar]
  5. Gossard F., Verly W. G. Properties of the main endonuclease specific for apurinic sites of Escherichia coli (endonuclease VI). Mechanism of apurinic site excision from DNA. Eur J Biochem. 1978 Jan 16;82(2):321–332. doi: 10.1111/j.1432-1033.1978.tb12026.x. [DOI] [PubMed] [Google Scholar]
  6. Laskey R. A. The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980;65(1):363–371. doi: 10.1016/s0076-6879(80)65047-2. [DOI] [PubMed] [Google Scholar]
  7. Mosbaugh D. W., Linn S. Further characterization of human fibroblast apurinic/apyrimidinic DNA endonucleases. The definition of two mechanistic classes of enzyme. J Biol Chem. 1980 Dec 25;255(24):11743–11752. [PubMed] [Google Scholar]
  8. Verly W. G., Gossard F., Crine P. In vitro repair of apurinic sites in DNA. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2273–2275. doi: 10.1073/pnas.71.6.2273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Weiss B. Endonuclease II of Escherichia coli is exonuclease III. J Biol Chem. 1976 Apr 10;251(7):1896–1901. [PubMed] [Google Scholar]

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