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. 1983 Dec 10;11(23):8369–8387. doi: 10.1093/nar/11.23.8369

Rapid synthesis of long-chain deoxyribooligonucleotides by the N-methylimidazolide phosphotriester method.

V A Efimov, A A Buryakova, S V Reverdatto, O G Chakhmakhcheva, Ovchinnikov YuA
PMCID: PMC326588  PMID: 6324083

Abstract

A modified phosphotriester method has been employed for the efficient chemical synthesis of long-chain deoxyribooligonucleotides. During the course of this work, a general and rapid procedure was developed for the preparation of 24-62-mers in solution. Preparative reversed phase column chromatography on silanized silica gel was used to purify triester intermediates starting from 10-mers. The rapid synthesis of 32-mer and 42-mer on glass and silica gel supports using suitably protected 2-8-mer blocks as coupling units has been also accomplished. In particular, a convenient procedure for the solid-phase synthesis of oligonucleotide blocks bearing 3'-terminal phosphodiester groups is described.

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Selected References

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