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. 1983 Dec 10;11(23):8431–8441. doi: 10.1093/nar/11.23.8431

Mass spectrometric monitoring of solid phase phosphate triester synthesis of DNA fragments.

L Alder, A Rosenthal, D Cech
PMCID: PMC326593  PMID: 6672769

Abstract

A new and convenient pyrolysis mass spectrometric approach for monitoring solid phase phosphate triester synthesis of oligodeoxyribonucleotides in 5'-3' direction has been developed. The base-typical fragment ions produced by pyrolysis at 280 degrees C of the polymer-bound oligonucleotide triesters in the mass spectrometer permit the analytical monitoring of DNA chain growth, using simple mathematical operations. The base composition can be determined directly from the polymer. In addition, minor nucleosides can easily be detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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