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. Author manuscript; available in PMC: 2013 Jan 10.
Published in final edited form as: Biochemistry. 2011 Dec 30;51(1):391–400. doi: 10.1021/bi201604b

FIGURE 4. Elevated Concentrations of Nucleotide Inhibit Terminase Strand Separation Activity.

FIGURE 4

Panel A. The strand separation assay was performed as described in Experimental Procedures except that IHF was omitted and the indicated concentration of nucleotide was included in the reaction mixture. Each data point represents the average of at least three separate experiments with standard deviation indicated with a bar. Panel B. The strand separation assay was performed in the absence of IHF and in the presence of 10 μM ADP. Additional nucleotides were added to a final concentration of 5 mM as indicated. Each bar represents the average of at least three separate experiments with standard deviation indicated.