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. 1981 Jan 10;9(1):19–30. doi: 10.1093/nar/9.1.19

Cloning of bovine growth hormone gene and its expression in bacteria.

E Keshet, A Rosner, Y Bernstein, M Gorecki, H Aviv
PMCID: PMC326665  PMID: 7010310

Abstract

A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the beta-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused beta-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH. Unlike beta-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.

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Selected References

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