Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Jan 10;9(1):47–53. doi: 10.1093/nar/9.1.47

The nucleotide sequence of spinach chloroplast tryptophan transfer RNA.

J Canaday, P Guillemaut, R Gloeckler, J H Weil
PMCID: PMC326667  PMID: 6907845

Abstract

Spinach chloroplast tRNATrp, purified by column chromatography and two-dimensional gel electrophoresis, has been sequenced using in vitro labeling techniques. The sequence is : pG-C-G-C-U-C-U-U-A-G-U-U-C-A-G-U-U-C-Gm-G-D-A-G-A-A-C-m2G-psi-G-G-G-psi-C-U-C-A-A*-A-A-C-C-C-G-A-U-G-N-C-G-U-A-G-G-T-psi-C-A-A-G-U-C-C-U-A-C-A-G-A-G-C-G-U-G -C-C-AOH. Like the E. coli suppressor tRNA psu+UGA which translates both the opal terminator codon U-G-A and the tryptophan codon U-G-G, spinach chloroplast tRNATrp has C-C-A as an anticodon and contains an A-U pair in the D-stem.

Full text

PDF
47

Images in this article

50Image
on p.50

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barrell B. G., Bankier A. T., Drouin J. A different genetic code in human mitochondria. Nature. 1979 Nov 8;282(5735):189–194. doi: 10.1038/282189a0. [DOI] [PubMed] [Google Scholar]
  2. Berkner K. L., Folk W. R. Polynucleotide kinase exchange reaction: quantitave assay for restriction endonuclease-generated 5'-phosphoroyl termini in DNA. J Biol Chem. 1977 May 25;252(10):3176–3184. [PubMed] [Google Scholar]
  3. Canaday J., Dirheimer G., Martin R. P. Yeast mitochondrial methionine initiator tRNA: characterization and nucleotide sequence. Nucleic Acids Res. 1980 Apr 11;8(7):1445–1457. doi: 10.1093/nar/8.7.1445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Canaday J., Guillemaut P., Weil J. H. The nucleotide sequences of the initiator transfer RNAs from bean cytoplasm and chloroplasts. Nucleic Acids Res. 1980 Mar 11;8(5):999–1008. doi: 10.1093/nar/8.5.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Driesel A. J., Crouse E. J., Gordon K., Bohnert H. J., Herrmann R. G., Steinmetz A., Mubumbila M., Keller M., Burkard G., Weil J. H. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA. Gene. 1979 Aug;6(4):285–306. doi: 10.1016/0378-1119(79)90070-2. [DOI] [PubMed] [Google Scholar]
  7. Eisenberg S. P., Soll L., Yarus M. The purification and sequence of a temperature-sensitive tryptophan tRNA. J Biol Chem. 1979 Jun 25;254(12):5562–5566. [PubMed] [Google Scholar]
  8. England T. E., Uhlenbeck O. C. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. doi: 10.1038/275560a0. [DOI] [PubMed] [Google Scholar]
  9. Fournier M., Labouesse J., Dirheimer G., Fix C., Keith G. Primary structure of bovine liver tRNATrp. Biochim Biophys Acta. 1978 Nov 21;521(1):198–208. doi: 10.1016/0005-2787(78)90262-9. [DOI] [PubMed] [Google Scholar]
  10. Fradin A., Gruhl H., Feldmann H. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Lett. 1975 Feb 1;50(2):185–189. doi: 10.1016/0014-5793(75)80485-6. [DOI] [PubMed] [Google Scholar]
  11. Harada F., Sawyer R. C., Dahlberg J. E. A primer ribonucleic acid for initiation of in vitro Rous sarcarcoma virus deoxyribonucleic acid synthesis. J Biol Chem. 1975 May 10;250(9):3487–3497. [PubMed] [Google Scholar]
  12. Hirsh D., Gold L. Translation of the UGA triplet in vitro by tryptophan transfer RNA's. J Mol Biol. 1971 Jun 14;58(2):459–468. doi: 10.1016/0022-2836(71)90363-9. [DOI] [PubMed] [Google Scholar]
  13. Hirsh D. Tryptophan transfer RNA as the UGA suppressor. J Mol Biol. 1971 Jun 14;58(2):439–458. doi: 10.1016/0022-2836(71)90362-7. [DOI] [PubMed] [Google Scholar]
  14. Keith G., Roy A., Ebel J. P., Dirheimer G. The primary structure of tryptophan transfer ribonucleic acid from brewer's yeast. II. Partial digestion with pancreatic ribonuclease and derivation of complete sequence. Biochimie. 1972;54(11):1417–1426. doi: 10.1016/s0300-9084(72)80083-x. [DOI] [PubMed] [Google Scholar]
  15. Keller M., Burkard G., Bohnert H. J., Mubumbila M., Gordon K., Steinmetz A., Heiser D., Crouse E. J., Weil J. H. Transfer RNA genes associated with the 16S and 23S rRNA genes of Euglena chloroplast DNA. Biochem Biophys Res Commun. 1980 Jul 16;95(1):47–54. doi: 10.1016/0006-291x(80)90702-0. [DOI] [PubMed] [Google Scholar]
  16. Macino G., Coruzzi G., Nobrega F. G., Li M., Tzagoloff A. Use of the UGA terminator as a tryptophan codon in yeast mitochondria. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3784–3785. doi: 10.1073/pnas.76.8.3784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  18. Sibler A. P., Bordonné R., Dirheimer G., Martin R. Structure primaire d'un tryptophane-tRNA de mitochondrie de levure capable de traduire le codon de terminaison U-G-A. C R Seances Acad Sci D. 1980 Mar 17;290(11):695–698. [PubMed] [Google Scholar]
  19. Silberklang M., Gillum A. M., RajBhandary U. L. The use of nuclease P1 in sequence analysis of end group labeled RNA. Nucleic Acids Res. 1977 Dec;4(12):4091–4108. doi: 10.1093/nar/4.12.4091. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  21. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
  22. Yaniv M., Folk W. R., Berg P., Soll L. A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG. J Mol Biol. 1974 Jun 25;86(2):245–260. doi: 10.1016/0022-2836(74)90016-3. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES