Abstract
On activated DNA aphidicolin competitively inhibits the incorporation of dCMP by both calf thymus DNA polymerase alpha A2 and C enzymes and inhibits the incorporation of the other three deoxynucleoside monophosphates apparently non-competitively. However, aphidicolin does not inhibit the incorporation of dAMP into poly(dT) . oligo(A)10 nor does it inhibit the incorporation of dGMP into poly(dC) . oligo(dG)10, but, it does competitively inhibit the incorporation of dTMP into poly(dA) . oligo(dT)10.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Habara A., Kano K., Nagano H., Mano Y., Ikegami S., Yamashita T. Inhibition of DNA synthesis in the adenovirus DNA replication complex by aphidicolin and 2',3'-dideoxythymidine triphosphate. Biochem Biophys Res Commun. 1980 Jan 15;92(1):8–12. doi: 10.1016/0006-291x(80)91511-9. [DOI] [PubMed] [Google Scholar]
- Hesslewood I. P., Holmes A. M., Wakeling W. F., Johnston I. R. Studies on the purification and properties of a 6.8-S DNA polymerase activity found in calf-thymus DNA polymerase-alpha fraction. Eur J Biochem. 1978 Mar;84(1):123–131. doi: 10.1111/j.1432-1033.1978.tb12148.x. [DOI] [PubMed] [Google Scholar]
- Holmer A. M., Hesslewood I. P., Johnston I. R. The occurrence of multiple activities in the high-molecular-weight DNA polymerase fraction of mammalian tissues. A preliminary study of some of their properties. Eur J Biochem. 1974 Apr 16;43(3):487–499. doi: 10.1111/j.1432-1033.1974.tb03436.x. [DOI] [PubMed] [Google Scholar]
- Ikegami S., Taguchi T., Ohashi M., Oguro M., Nagano H., Mano Y. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha. Nature. 1978 Oct 5;275(5679):458–460. doi: 10.1038/275458a0. [DOI] [PubMed] [Google Scholar]
- Krokan H., Schaffer P., DePamphilis M. L. Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid. Biochemistry. 1979 Oct 2;18(20):4431–4443. doi: 10.1021/bi00587a025. [DOI] [PubMed] [Google Scholar]
- McKune K., Holmes A. M. Further studies on partially purified calf thymus DNA polymerase a. Nucleic Acids Res. 1979 Jul 25;6(10):3341–3352. doi: 10.1093/nar/6.10.3341. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McKune K., Holmes A. M. Studies on the processivity of highly purified calf thymus 8S and 7.3S DNA polymerase alpha. Biochem Biophys Res Commun. 1979 Oct 12;90(3):864–890. doi: 10.1016/0006-291x(79)91907-7. [DOI] [PubMed] [Google Scholar]
- Oguro M., Shioda M., Nagano H., Mano Y., Hanaoka F., Yamada M. The mode of action of aphidicolin on DNA synthesis in isolated nuclei. Biochem Biophys Res Commun. 1980 Jan 15;92(1):13–19. doi: 10.1016/0006-291x(80)91512-0. [DOI] [PubMed] [Google Scholar]
- Oguro M., Suzuki-Hori C., Nagano H., Mano Y., Ikegami S. The mode of inhibitory action by aphidicolin on eukaryotic DNA polymerase alpha. Eur J Biochem. 1979 Jul;97(2):603–607. doi: 10.1111/j.1432-1033.1979.tb13149.x. [DOI] [PubMed] [Google Scholar]
- Okura A., Yoshida S. Differential inhibition of DNA polymerases of calf thymus by 9-beta-D-arabinofuranosyladenine-5'-triphosphate. J Biochem. 1978 Sep;84(3):727–732. doi: 10.1093/oxfordjournals.jbchem.a132178. [DOI] [PubMed] [Google Scholar]
- Pedrali-Noy G., Spadari S. Effect of aphidicolin on viral and human DNA polymerases. Biochem Biophys Res Commun. 1979 Jun 27;88(4):1194–1202. doi: 10.1016/0006-291x(79)91106-9. [DOI] [PubMed] [Google Scholar]
- Wright G. E., Baril E. F., Brown N. C. Butylanilinouracil: a selective inhibitor of HeLa cell DNA synthesis and HeLa cell DNA polymerase alpha. Nucleic Acids Res. 1980 Jan 11;8(1):99–109. doi: 10.1093/nar/8.1.99. [DOI] [PMC free article] [PubMed] [Google Scholar]