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. 1981 Feb 25;9(4):1005–1018. doi: 10.1093/nar/9.4.1005

On the mechanism of inhibitory effect of violamycin antibiotics on the transcription by bacteriophage T3-induced RNA polymerase.

B Brux, D Herrmann, G Richter, W F Fleck, D G Strauss, W Koch
PMCID: PMC326729  PMID: 7232204

Abstract

The effect of three components of the anthracycline antibiotic violamycin on the transcription of bacteriophage T3 DNA by bacteriophage T3-induced RNA polymerase has been investigated in a cell-free system. The glycosides of violamycin BI possess the highest inhibitory activity, whereas those of violamycin BII and violamycin A show a reduced inhibitory effect. Concentrations of violamycin BI depressing the incorporation of (3H)UMP into RNA chains have only a slight effect on the binding of the T3 RNA polymerase to T3 DNA and on the incorporation of GTP as the first nucleotide. This shows that the primary target of the antibiotic is not the initiation of the RNA synthesis. The binding of violamycin BI to T3 DNA causes a strong reduction of the elongation rate of the RNA chains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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