Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Mar 25;9(6):1351–1364. doi: 10.1093/nar/9.6.1351

A putative precursor for the small ribosomal RNA from mitochondria of Saccharomyces cerevisiae.

K A Osinga, R F Evers, J C Van der Laan, H F Tabak
PMCID: PMC326762  PMID: 6262728

Abstract

We have characterized a putative precursor RNA (15.5S) for the 15S ribosomal RNA in mitochondria of Saccharomyces cerevisiae. Hybrids were formed with mitochondrial RNA and mtDNA fragments terminally labelled at restriction sites located within the gene coding for 15S ribosomal RNA and treated with S1 nuclease (Berk, A.J. and Sharp, J.A. (1977) 12, 721-732). Sites of resistant hybrids were measured by agarose gel electrophoresis and end points of RNAs determined. The 15.5S RNA is approximately 80 nucleotides longer than the 15S ribosomal RNA, with the extra sequences being located at the 5'-end. Both 15S ribosomal RNA and 15.5S RNA are fully localised within a 2000 base pair HapII fragment. This putative precursor and the mature 15S ribosomal RNA are also found in petite mutants which retain the 15S ribosomal RNA gene. The petite mutant with the smallest genetic complexity has its end point of deletion (junction) just outside the HapII site located in the 5' flank of the 15S ribosomal RNA genes as determined by S1 nuclease analysis. This leaves a DNA stretch approximately 300 base pairs long where an initiation signal for mitochondrial transcription may be present.

Full text

PDF
1351

Images in this article

1355Image
on p.1355
1357Image
on p.1357
1358Image
on p.1358
1359Image
on p.1359
1360Image
on p.1360
1361Image
on p.1361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnberg A. C., Van Ommen G. J., Grivell L. A., Van Bruggen E. F., Borst P. Some yeast mitochondrial RNAs are circular. Cell. 1980 Feb;19(2):313–319. doi: 10.1016/0092-8674(80)90505-x. [DOI] [PubMed] [Google Scholar]
  2. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  3. Bos J. L., Osinga K. A., Van der Horst G., Hecht N. B., Tabak H. F., Van Ommen G. J., Borst P. Splice point sequence and transcripts of the intervening sequence in the mitochondrial 21S ribosomal RNA gene of yeast. Cell. 1980 May;20(1):207–214. doi: 10.1016/0092-8674(80)90248-2. [DOI] [PubMed] [Google Scholar]
  4. Dujon B. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci. Cell. 1980 May;20(1):185–197. doi: 10.1016/0092-8674(80)90246-9. [DOI] [PubMed] [Google Scholar]
  5. Fox T. D. Genetic and physical analysis of the mitochondrial gene for subunit II of yeast cytochrome c oxidase. J Mol Biol. 1979 May 5;130(1):63–82. doi: 10.1016/0022-2836(79)90552-7. [DOI] [PubMed] [Google Scholar]
  6. Goldbach R. W., Borst P., Bollen-de Boer J. E., van Bruggen E. F. The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST. Biochim Biophys Acta. 1978 Nov 21;521(1):169–186. doi: 10.1016/0005-2787(78)90260-5. [DOI] [PubMed] [Google Scholar]
  7. Grivell L. A., Reijnders L., Borst P. Isolation of yeast mitochondrial ribosomes highly active in protein synthesis. Biochim Biophys Acta. 1971 Sep 30;247(1):91–103. doi: 10.1016/0005-2787(71)90811-2. [DOI] [PubMed] [Google Scholar]
  8. Jeffreys A. J., Flavell R. A. A physical map of the DNA regions flanking the rabbit beta-globin gene. Cell. 1977 Oct;12(2):429–439. doi: 10.1016/0092-8674(77)90119-2. [DOI] [PubMed] [Google Scholar]
  9. Merten S., Synenki R. M., Locker J., Christianson T., Rabinowitz M. Processing of precursors of 21S ribosomal RNA from yeast mitochondria. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1417–1421. doi: 10.1073/pnas.77.3.1417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moorman A. F., Grivell L. A., Lamie F., Smits H. L. Identification of mitochondrial gene products by DNA-directed protein synthesis in vitro. Biochim Biophys Acta. 1978 Apr 27;518(2):351–365. doi: 10.1016/0005-2787(78)90192-2. [DOI] [PubMed] [Google Scholar]
  11. Morimoto R., Locker J., Synenki R. M., Rabinowitz M. Transcription, processing, and mapping of mitochondrial RNA from grande and petite yeast. J Biol Chem. 1979 Dec 25;254(24):12461–12470. [PubMed] [Google Scholar]
  12. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  13. Perry R. P. Processing of RNA. Annu Rev Biochem. 1976;45:605–629. doi: 10.1146/annurev.bi.45.070176.003133. [DOI] [PubMed] [Google Scholar]
  14. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  15. Sanders J. P., Heyting C., Borst P. The organization of genes in yeast mitochondrial DNA. I. The genes for large and small ribosomal RNA are far apart. Biochem Biophys Res Commun. 1975 Jul 22;65(2):699–707. doi: 10.1016/s0006-291x(75)80202-6. [DOI] [PubMed] [Google Scholar]
  16. Sanders J. P., Weijers P. J., Groot G. S., Borst P. Properties of mitochondrial DNA from Kluyveromyces lactis. Biochim Biophys Acta. 1974 Dec 6;374(2):136–144. doi: 10.1016/0005-2787(74)90357-8. [DOI] [PubMed] [Google Scholar]
  17. Seeburg P. H., Shine J., Martial J. A., Ullrich A., Baxter J. D., Goodman H. M. Nucleotide sequence of part of the gene for human chorionic somatomammotropin: purification of DNA complementary to predominant mRNA species. Cell. 1977 Sep;12(1):157–165. doi: 10.1016/0092-8674(77)90193-3. [DOI] [PubMed] [Google Scholar]
  18. Tabak H. F., Flavell R. A. A method for the recovery of DNA from agarose gels. Nucleic Acids Res. 1978 Jul;5(7):2321–2332. doi: 10.1093/nar/5.7.2321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Ommen G. J., Groot G. S., Grivell L. A. Transcription maps of mtDNAs of two strains of saccharomyces: transcription of strain-specific insertions; Complex RNA maturation and splicing. Cell. 1979 Oct;18(2):511–523. doi: 10.1016/0092-8674(79)90068-0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES