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. 1981 Mar 25;9(6):1445–1450. doi: 10.1093/nar/9.6.1445

Nucleotide sequence of 5S ribosomal RNA from Aspergillus nidulans and Neurospora crassa.

B Piechulla, U Hahn, L W McLaughlin, H Küntzel
PMCID: PMC326768  PMID: 6453331

Abstract

The nucleotide sequences of 5S rRNA molecules isolated from the cytosol and the mitochondria of the ascomycetes A. nidulans and N. crassa were determined by partial chemical cleavage of 3'-terminally labelled RNA. The sequence identity of the cytosolic and mitochondrial RNA preparations confirms the absence of mitochondrion-specific 5S rRNA in these fungi. The sequences of the two organisms differ in 35 positions, and each sequence differs from yeast 5S rRNA in 44 positions. Both molecules contain the sequence GCUC in place of GAAC or GAUY found in all other 5S rRNAs, indicating that this region is not universally involved in base-pairing to the invariant GTpsiC sequence of tRNAs.

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Selected References

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  1. Arcari P., Brownlee G. G. The nucleotide sequence of a small (3S) seryl-tRNA (anticodon GCU) from beef heart mitochondria. Nucleic Acids Res. 1980 Nov 25;8(22):5207–5212. doi: 10.1093/nar/8.22.5207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baer R. J., Dubin D. T. The sequence of a possible 5S RNA-equivalent in hamster mitochondria. Nucleic Acids Res. 1980 Aug 25;8(16):3603–3610. doi: 10.1093/nar/8.16.3603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Benhamou J., Jourdan R., Jordan B. R. Sequence of Drosophila 5S RNA synthesized by cultured cells and by the insect at different developmental stages. Homogeneity of the product and homologies with other 5S RNA's at the level of primary and secondary structure. J Mol Evol. 1977 May 13;9(3):279–298. doi: 10.1007/BF01796116. [DOI] [PubMed] [Google Scholar]
  4. Borst P., Grivell L. A. The mitochondrial genome of yeast. Cell. 1978 Nov;15(3):705–723. doi: 10.1016/0092-8674(78)90257-x. [DOI] [PubMed] [Google Scholar]
  5. Dubin D. T., Friend D. A. Comparison of cytoplasmic and mitochondrial 4 S RNA from cultured hamster cells: physical and metabolic properties. J Mol Biol. 1972 Nov 14;71(2):163–175. doi: 10.1016/0022-2836(72)90344-0. [DOI] [PubMed] [Google Scholar]
  6. Edelman M., Verma I. M., Littauer U. Z. Mitochondrial ribosomal RNA from Aspergillus nidulans: characterization of a novel molecular species. J Mol Biol. 1970 Apr 14;49(1):67–83. doi: 10.1016/0022-2836(70)90376-1. [DOI] [PubMed] [Google Scholar]
  7. Erdmann V. A. Collection of published 5S and 5.8S RNA sequences and their precursors. Nucleic Acids Res. 1979 Jan;6(1):r29–r44. doi: 10.1093/nar/6.1.419-c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Erdmann V. A., Sprinzl M., Pongs O. The involvement of 5S RNA in the binding of tRNA to ribosomes. Biochem Biophys Res Commun. 1973 Oct 1;54(3):942–948. doi: 10.1016/0006-291x(73)90785-7. [DOI] [PubMed] [Google Scholar]
  9. Erdmann V. A. Structure and function of 5S and 5.8 S RNA. Prog Nucleic Acid Res Mol Biol. 1976;18:45–90. [PubMed] [Google Scholar]
  10. Fox G. E., Woese C. R. The architecture of 5S rRNA and its relation to function. J Mol Evol. 1975 Oct 3;6(1):61–76. doi: 10.1007/BF01732674. [DOI] [PubMed] [Google Scholar]
  11. Küntzel H., Noll H. Mitochondrial and cytoplasmic polysomes from Neurospora crassa. Nature. 1967 Sep 23;215(5108):1340–1345. doi: 10.1038/2151340a0. [DOI] [PubMed] [Google Scholar]
  12. Lizardi P. M., Luck D. J. Absence of a 5S RNA complnent in the mitochondrial ribosomes of Neurospora crassa. Nat New Biol. 1971 Feb 3;229(5):140–142. doi: 10.1038/newbio229140a0. [DOI] [PubMed] [Google Scholar]
  13. Peattie D. A. Direct chemical method for sequencing RNA. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760–1764. doi: 10.1073/pnas.76.4.1760. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rubin G. M. Preparation of RNA and ribosomes from yeast. Methods Cell Biol. 1975;12:45–64. doi: 10.1016/s0091-679x(08)60951-6. [DOI] [PubMed] [Google Scholar]
  15. Schäfer K. P., Bugge G., Grandi M., Küntzel H. Transcription of mitochondrial DNA in vitro from Neurospora crassa. Eur J Biochem. 1971 Aug 25;21(4):478–488. doi: 10.1111/j.1432-1033.1971.tb01493.x. [DOI] [PubMed] [Google Scholar]
  16. Stepień P. P., Bernard U., Cooke H. J., Küntzel H. Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans. Nucleic Acids Res. 1978 Feb;5(2):317–330. doi: 10.1093/nar/5.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sugino A., Snoper T. J., Cozzarelli N. R. Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates. J Biol Chem. 1977 Mar 10;252(5):1732–1738. [PubMed] [Google Scholar]
  18. de Bruijn M. H., Schreier P. H., Eperon I. C., Barrell B. G., Chen E. Y., Armstrong P. W., Wong J. F., Roe B. A. A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem. Nucleic Acids Res. 1980 Nov 25;8(22):5213–5222. doi: 10.1093/nar/8.22.5213. [DOI] [PMC free article] [PubMed] [Google Scholar]

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