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. 1981 Apr 10;9(7):1591–1597. doi: 10.1093/nar/9.7.1591

Vertebrate histone gene transcription occurs from both DNA strands.

S Bruschi, J R Wells
PMCID: PMC326784  PMID: 7232222

Abstract

Three clones containing vertebrate histone genes, two from chicken and one from human have been investigated. DNA from each recombinant within lambda Charon 4A has been strand-separated on agarose gels in the presence of poly r(UG). Southern transfer analysis with histone cDNA indicates that in each case transcription occurs from both strands. These results argue against the possibility of polycistronic transcription of these vertebrate histone genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crawford R. J., Krieg P., Harvey R. P., Hewish D. A., Wells J. R. Histone genes are clustered with a 15-kilobase repeat in the chicken genome. Nature. 1979 May 10;279(5709):132–136. doi: 10.1038/279132a0. [DOI] [PubMed] [Google Scholar]
  2. Crawford R. J., Wells J. R. Short half-life precursor of globin messenger RNA from chicken erythroblasts. Biochemistry. 1978 May 2;17(9):1591–1596. doi: 10.1021/bi00602a002. [DOI] [PubMed] [Google Scholar]
  3. Goldbach R. W., Evers R. F., Borst P. Electrophoretic strand separation of long DNAs with poly (U,G) in agarose gels. Nucleic Acids Res. 1978 Aug;5(8):2743–2754. doi: 10.1093/nar/5.8.2743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gross K., Schaffner W., Telford J., Birnstiel M. Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences. Cell. 1976 Aug;8(4):479–484. doi: 10.1016/0092-8674(76)90215-4. [DOI] [PubMed] [Google Scholar]
  5. Hackett P. B., Traub P., Gallwitz D. The histone genes in HeLa cells are on individual transcriptional units. J Mol Biol. 1978 Dec 25;126(4):619–635. doi: 10.1016/0022-2836(78)90012-8. [DOI] [PubMed] [Google Scholar]
  6. Harvey R. P., Wells J. R. Isolation of a genomal clone containing chicken histone genes. Nucleic Acids Res. 1979 Dec 11;7(7):1787–1798. doi: 10.1093/nar/7.7.1787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hentschel C., Irminger J. C., Bucher P., Birnstiel M. L. Sea urchin histone mRNA termini are located in gene regions downstream from putative regulatory sequences. Nature. 1980 May 15;285(5761):147–151. doi: 10.1038/285147a0. [DOI] [PubMed] [Google Scholar]
  8. Hereford L., Fahrner K., Woolford J., Jr, Rosbash M., Kaback D. B. Isolation of yeast histone genes H2A and H2B. Cell. 1979 Dec;18(4):1261–1271. doi: 10.1016/0092-8674(79)90237-x. [DOI] [PubMed] [Google Scholar]
  9. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  10. Kornberg R. D. Structure of chromatin. Annu Rev Biochem. 1977;46:931–954. doi: 10.1146/annurev.bi.46.070177.004435. [DOI] [PubMed] [Google Scholar]
  11. Lifton R. P., Goldberg M. L., Karp R. W., Hogness D. S. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1047–1051. doi: 10.1101/sqb.1978.042.01.105. [DOI] [PubMed] [Google Scholar]
  12. Melli M., Spinelli G., Wyssling H., Arnold E. Presence of histone mRNA sequences in high molecular weight RNA of HeLa cells. Cell. 1977 Jul;11(3):651–661. doi: 10.1016/0092-8674(77)90082-4. [DOI] [PubMed] [Google Scholar]
  13. Perlman D., Huberman J. A. Preparation of large quantities of separated strands from simian virus 40 DNA restriction fragments by low-temperature low-salt agarose gel electrophoresis. Anal Biochem. 1977 Dec;83(2):666–677. doi: 10.1016/0003-2697(77)90071-9. [DOI] [PubMed] [Google Scholar]
  14. Richards R. I., Wells J. R. Chicken globin genes. Nucleotide sequence of cDNA clones coding for the alpha-globin expressed during hemolytic anemia. J Biol Chem. 1980 Oct 10;255(19):9306–9311. [PubMed] [Google Scholar]
  15. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  16. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]

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