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. 1981 Apr 10;9(7):1691–1706. doi: 10.1093/nar/9.7.1691

Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.

M L Duckworth, M J Gait, P Goelet, G F Hong, M Singh, R C Titmas
PMCID: PMC326791  PMID: 7232224

Abstract

Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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