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. 1981 Apr 24;9(8):1933–1939. doi: 10.1093/nar/9.8.1933

5-(carboxymethylaminomethyl)-2-thiouridine, a new modified nucleoside found at the first letter position of the anticodon.

Y Yamada, K Murao, H Ishikura
PMCID: PMC326813  PMID: 7017599

Abstract

The structure of a modified uridine derivative which was detected at the first letter position of the anticodon of Bacillus subtilis tRNA1Lys was determined to be 5-(carboxymethylaminomethyl)-2-thiouridine. The determination was mainly based in this ultraviolet absorption spectra and mass spectrometric analysis of the trimethylsilyl derivative.

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1933

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carbon J., David H., Studier M. H. Thiobases in Escherchia coli Transfer RNA: 2-Thiocytosine and 5-Methylaminomethyl-2-thiouracil. Science. 1968 Sep 13;161(3846):1146–1147. doi: 10.1126/science.161.3846.1146. [DOI] [PubMed] [Google Scholar]
  2. Chakraburtty K., Steinschneider A., Case R. V., Mehler A. H. Primary structure of tRNA-Lys of E. coli B. Nucleic Acids Res. 1975 Nov;2(11):2069–2075. doi: 10.1093/nar/2.11.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Murao K., Hasegawa T., Ishikura H. 5-methoxyuridine: a new minor constituent located in the first position of the anticodon of tRNAAla, tRNAThr, and tRNAVal from Bacillus subtilis. Nucleic Acids Res. 1976 Oct;3(10):2851–2860. doi: 10.1093/nar/3.10.2851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nishimura S. Minor components in transfer RNA: their characterization, location, and function. Prog Nucleic Acid Res Mol Biol. 1972;12:49–85. [PubMed] [Google Scholar]
  5. Saneyoshi M., Nishimura S. Selective modification of 4-thiouridylate residue in Escherichia coli transfer RNA with cyanogen bromide. Biochim Biophys Acta. 1970 Apr 15;204(2):389–399. doi: 10.1016/0005-2787(70)90158-9. [DOI] [PubMed] [Google Scholar]
  6. Sen G. C., Ghosh H. P. Role of modified nucleosides in tRNA: effect of modification of the 2-thiouridine derivative located at the 5'-end of the anticodon of yeast transfer RNA Lys2. Nucleic Acids Res. 1976 Mar;3(3):523–535. doi: 10.1093/nar/3.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Yamada Y., Ishikura H. Nucleotide sequence of a lysine tRNA from Bacillus subtilis. Nucleic Acids Res. 1977 Dec;4(12):4291–4303. doi: 10.1093/nar/4.12.4291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Yoshida M., Takeishi K., Ukita T. Structural studies on a yeast glutamic acid tRNA specific to GAA codon. Biochim Biophys Acta. 1971 Jan 1;228(1):153–166. doi: 10.1016/0005-2787(71)90555-7. [DOI] [PubMed] [Google Scholar]

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