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. 1981 Apr 24;9(8):1941–1947. doi: 10.1093/nar/9.8.1941

Sequence of DNA complementary to a small RNA segment of influenza virus A/NT/60/68.

B A Moss, G G Brownlee
PMCID: PMC326814  PMID: 7243594

Abstract

A small RNA segment from the influenza virus strain A/NT/60/68 (H3N2) was converted to cDNA and then to double-stranded DNA using synthetic oligodeoxynucleotide primers. The double-stranded form was cloned into the bacteriophage M1 3mp7. Clones yielding single-strand recombinant templates in opposite orientation were sequenced by the Sanger dideoxynucleotide chain termination technique. The small viral RNA was 422 nucleotides long and the evidence indicated that it was formed by internal deletion of segment 3. It also contained sequences homologous to segment 1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Air G. M. Nucleotide sequence coding for the "signal peptide" and N terminus of the hemagglutinin from an asian (H2N2) strain of influenza virus. Virology. 1979 Sep;97(2):468–472. doi: 10.1016/0042-6822(79)90358-1. [DOI] [PubMed] [Google Scholar]
  2. Anderson S., Gait M. J., Mayol L., Young I. G. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2. Nucleic Acids Res. 1980 Apr 25;8(8):1731–1743. doi: 10.1093/nar/8.8.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Both G. W., Air G. M. Nucleotide sequence coding for the N-terminal region of the matrix protein influenza virus. Eur J Biochem. 1979 May 15;96(2):363–372. doi: 10.1111/j.1432-1033.1979.tb13048.x. [DOI] [PubMed] [Google Scholar]
  4. Catterall J. F., O'Malley B. W., Robertson M. A., Staden R., Tanaka Y., Brownlee G. G. Nucleotide sequence homology at 12 intron--exon junctions in the chick ovalbumin gene. Nature. 1978 Oct 12;275(5680):510–513. doi: 10.1038/275510a0. [DOI] [PubMed] [Google Scholar]
  5. Crumpton W. M., Dimmock N. J., Minor P. D., Avery R. J. The RNAs of defective-interfering influenza virus. Virology. 1978 Oct 15;90(2):370–373. doi: 10.1016/0042-6822(78)90322-7. [DOI] [PubMed] [Google Scholar]
  6. Davis A. R., Hiti A. L., Nayak D. P. Influenza defective interfering viral RNA is formed by internal deletion of genomic RNA. Proc Natl Acad Sci U S A. 1980 Jan;77(1):215–219. doi: 10.1073/pnas.77.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Davis A. R., Nayak D. P. Sequence relationships among defective interfering influenza viral RNAs. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3092–3096. doi: 10.1073/pnas.76.7.3092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Desselberger U., Racaniello V. R., Zazra J. J., Palese P. The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene. 1980 Feb;8(3):315–328. doi: 10.1016/0378-1119(80)90007-4. [DOI] [PubMed] [Google Scholar]
  9. Dohner D., Monroe S., Weiss B., Schlesinger S. Oligonucleotide mapping studies of standard and defective Sindbis virus RNA. J Virol. 1979 Feb;29(2):794–798. doi: 10.1128/jvi.29.2.794-798.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fazekas de St Groth New criteria for the selection of influenza vaccine strains. Bull World Health Organ. 1969;41(3):651–657. [PMC free article] [PubMed] [Google Scholar]
  11. Hamlyn P. H., Browniee G. G., Cheng C. C., Gait M. J., Milstein C. Complete sequence of constant and 3' noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing. Cell. 1978 Nov;15(3):1067–1075. doi: 10.1016/0092-8674(78)90290-8. [DOI] [PubMed] [Google Scholar]
  12. Heidecker G., Messing J., Gronenborn B. A versatile primer for DNA sequencing in the M13mp2 cloning system. Gene. 1980 Jun;10(1):69–73. doi: 10.1016/0378-1119(80)90145-6. [DOI] [PubMed] [Google Scholar]
  13. Janda J. M., Davis A. R., Nayak D. P., De B. K. Diversity and generation of defective interfering influenza virus particles. Virology. 1979 May;95(1):48–58. doi: 10.1016/0042-6822(79)90400-8. [DOI] [PubMed] [Google Scholar]
  14. Janda J. M., Nayak D. P. Defective influenza viral ribonucleoproteins cause interference. J Virol. 1979 Nov;32(2):697–702. doi: 10.1128/jvi.32.2.697-702.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kennedy S. I. Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. J Mol Biol. 1976 Dec;108(2):491–511. doi: 10.1016/s0022-2836(76)80132-5. [DOI] [PubMed] [Google Scholar]
  17. Leppert M., Kort L., Kolakofsky D. Further characterization of Sendai virus DI-RNAs: a model for their generation. Cell. 1977 Oct;12(2):539–552. doi: 10.1016/0092-8674(77)90130-1. [DOI] [PubMed] [Google Scholar]
  18. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Nakajima K., Ueda M., Sugiura A. Origin of small RNA in von Magnus particles of influenza virus. J Virol. 1979 Mar;29(3):1142–1148. doi: 10.1128/jvi.29.3.1142-1148.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robertson H. D., Dickson E., Plotch S. J., Krug R. M. Identification of the RNA region transferred from a representative primer, beta-globin mRNA, to influenza mRNA during in vitro transcription. Nucleic Acids Res. 1980 Mar 11;8(5):925–942. doi: 10.1093/nar/8.5.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schubert M., Keene J. D., Lazzarini R. A., Emerson S. U. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–112. doi: 10.1016/0092-8674(78)90086-7. [DOI] [PubMed] [Google Scholar]
  24. Semler B. L., Perrault J., Abelson J., Holland J. J. Sequence of a RNA templated by the 3'-OH RNA terminus of defective interfering particles of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4704–4708. doi: 10.1073/pnas.75.10.4704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  26. Skehel J. J., Hay A. J. Nucleotide sequences at the 5' termini of influenza virus RNAs and their transcripts. Nucleic Acids Res. 1978 Apr;5(4):1207–1219. doi: 10.1093/nar/5.4.1207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sleigh M. J., Both G. W., Brownlee G. G. A new method for the size estimation of the RNA genome segments of influenza virus. Nucleic Acids Res. 1979 Apr;6(4):1309–1321. doi: 10.1093/nar/6.4.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stark C., Kennedy S. I. The generation and propagation of defective-interfering particles of Semliki Forest virus in different cell types. Virology. 1978 Aug;89(1):285–299. doi: 10.1016/0042-6822(78)90060-0. [DOI] [PubMed] [Google Scholar]
  29. Ueda M., Nakajima K., Sugiura A. Extra RNAs of von Magnus particles of influenza virus cause reduction of particular polymerase genes. J Virol. 1980 Apr;34(1):1–8. doi: 10.1128/jvi.34.1.1-8.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. VON MAGNUS P. Incomplete forms of influenza virus. Adv Virus Res. 1954;2:59–79. doi: 10.1016/s0065-3527(08)60529-1. [DOI] [PubMed] [Google Scholar]
  31. Winter G., Fields S. Cloning of influenza cDNA ino M13: the sequence of the RNA segment encoding the A/PR/8/34 matrix protein. Nucleic Acids Res. 1980 May 10;8(9):1965–1974. doi: 10.1093/nar/8.9.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Winter G., Fields S., Gait M. J., Brownlee G. G. The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment of 8 influenza virus (A/PR/8/34). Nucleic Acids Res. 1981 Jan 24;9(2):237–245. doi: 10.1093/nar/9.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]

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