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. 1981 Jun 25;9(12):2777–2790. doi: 10.1093/nar/9.12.2777

Cloning of multiple copies of immunoglobulin variable kappa genes in cosmid vectors.

R Cattaneo, J Gorski, B Mach
PMCID: PMC326892  PMID: 6269060

Abstract

The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variable region probe were identified. In 9 of these recombinants, the size of the insert ranges from 30 to 43 kilobases. Factors affecting the cloning efficiency of a complex mammalian genome in cosmids were studied. The stability of these recombinant cosmids and the preparation of recombinant cosmid DNA are also discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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