Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1981 Jun 25;9(12):2807–2817. doi: 10.1093/nar/9.12.2807

Synthesis of oligodeoxyribonucleotides on silica gel support

Flora Chow 1, Tomas Kempe 1, Gunnar Palm 1
PMCID: PMC326895  PMID: 6269061

Abstract

A rapid solid phase method of oligonucleotide synthesis based on monomeric protected nucleosides has been developed.

Full text

PDF
2807

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Caruthers M. H., Beaucage S. L., Efcavitch J. W., Fisher E. F., Matteucci M. D., Stabinsky Y. New chemical methods for synthesizing polynucleotides. Nucleic Acids Symp Ser. 1980;(7):215–223. [PubMed] [Google Scholar]
  2. Daub G. W., van Tamelen E. E. Synthesis of oligoribonucleotides based on the facile cleavage of methyl phosphotriester intermediates. J Am Chem Soc. 1977 May 11;99(10):3526–3528. doi: 10.1021/ja00452a069. [DOI] [PubMed] [Google Scholar]
  3. Fritz H. J., Belagaje R., Brown E. L., Fritz R. H., Jones R. A., Lees R. G., Khorana H. G. High-pressure liquid chromatography in polynucleotide synthesis. Biochemistry. 1978 Apr 4;17(7):1257–1267. doi: 10.1021/bi00600a020. [DOI] [PubMed] [Google Scholar]
  4. Gait M. J., Singh M., Sheppard R. C., Edge M. D., Greene A. R., Heathcliffe G. R., Atkinson T. C., Newton C. R., Markham A. F. Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates. Nucleic Acids Res. 1980 Mar 11;8(5):1081–1096. doi: 10.1093/nar/8.5.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Köster H., Hoppe N., Kohli V., Kröpelin M., Kaut H., Kulikowski K. Some improvements in the synthesis of DNA of biological interest. Nucleic Acids Symp Ser. 1980;(7):39–60. [PubMed] [Google Scholar]
  6. Letsinger R. L., Lunsford W. B. Synthesis of thymidine oligonucleotides by phosphite triester intermediates. J Am Chem Soc. 1976 Jun 9;98(12):3655–3661. doi: 10.1021/ja00428a045. [DOI] [PubMed] [Google Scholar]
  7. Markham A. F., Edge M. D., Atkinson T. C., Greene A. R., Heathcliffe G. R., Newton C. R., Scanlon D. Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support. Nucleic Acids Res. 1980 Nov 25;8(22):5193–5205. doi: 10.1093/nar/8.22.5193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Narang S. A., Brousseau R., Hsiung H. M., Michniewicz J. J. Chemical synthesis of deoxyoligonucleotides by the modified triester method. Methods Enzymol. 1980;65(1):610–620. doi: 10.1016/s0076-6879(80)65063-0. [DOI] [PubMed] [Google Scholar]
  9. Tu C. P., Wu R. Sequence analysis of short DNA fragments. Methods Enzymol. 1980;65(1):620–638. [PubMed] [Google Scholar]
  10. Weber H., Khorana H. G. CIV. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. Chemical synthesis of an icosadeoxynucleotide corresponding to the nucleotide sequence 21 to 40. J Mol Biol. 1972 Dec 28;72(2):219–249. doi: 10.1016/0022-2836(72)90147-7. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES