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. 1982 Oct 25;10(20):6353–6362. doi: 10.1093/nar/10.20.6353

Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.

R Contreras, H Cheroutre, W Degrave, W Fiers
PMCID: PMC326924  PMID: 6294601

Abstract

A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wheat germ cell-free protein synthesizing systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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