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. 1982 Oct 25;10(20):6383–6387. doi: 10.1093/nar/10.20.6383

The nucleotide sequence of 5.8S rRNA from the posterior silk gland of the silkworm Philosamia cynthia ricini.

Y X Feng, G Krupp, H J Gross
PMCID: PMC326927  PMID: 7177850

Abstract

The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence in text). This is one of the largest known 5.8S rRNAs. As compared to Bombyx 5.8S rRNA, it is two nucleotides longer; two nucleotides near the 5'end and two nucleotides near the 3'end are different, and psi 61 of the Bombyx RNA sequence is an unmodified U in Philosamia RNA. The secondary structure of Philosamia 5.8S rRNA may differ from the Bombyx RNA structure by three additional base pairs at the 5'/3' ends.

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Selected References

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  1. Darlix J. L., Rochaix J. D. Nucleotide sequence and structure of cytoplasmic 5S RNA and 5.8S RNA of Chlamydomonas reinhardii. Nucleic Acids Res. 1981 Mar 25;9(6):1291–1299. doi: 10.1093/nar/9.6.1291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. England T. E., Bruce A. G., Uhlenbeck O. C. Specific labeling of 3' termini of RNA with T4 RNA ligase. Methods Enzymol. 1980;65(1):65–74. doi: 10.1016/s0076-6879(80)65011-3. [DOI] [PubMed] [Google Scholar]
  3. Erdmann V. A. Collection of published 5S and 5.8S RNA sequences and their precursors. Nucleic Acids Res. 1982 Jan 22;10(2):r93–115. doi: 10.1093/nar/10.2.762-c. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fujiwara H., Kawata Y., Ishikawa H. Primary and secondary structure of 5.8S rRNA from the silkgland of Bombyx mori. Nucleic Acids Res. 1982 Apr 10;10(7):2415–2418. doi: 10.1093/nar/10.7.2415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gross H. J., Krupp G., Domdey H., Raba M., Jank P., Lossow C., Alberty H., Ramm K., Sänger H. L. Nucleotide sequence and secondary structure of citrus exocortis and chrysanthemum stunt viroid. Eur J Biochem. 1982 Jan;121(2):249–257. doi: 10.1111/j.1432-1033.1982.tb05779.x. [DOI] [PubMed] [Google Scholar]
  6. Ishikawa H. Re-joining of the 18S fragments dissociated from the 28S ribosomal RNA of insect: a structural role of 5.8S RNA. Biochem Biophys Res Commun. 1979 Sep 27;90(2):417–424. doi: 10.1016/0006-291x(79)91251-8. [DOI] [PubMed] [Google Scholar]
  7. Jordan B. R., Latil-Damotte M., Jourdan R. Coding and spacer sequences in the 5.8S-2S region of Sciara coprophila ribosomal DNA. Nucleic Acids Res. 1980 Aug 25;8(16):3565–3573. doi: 10.1093/nar/8.16.3565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King H. W., Gould H. Low molecular weight ribonucleic acid in rabbit reticulocyte ribosomes. J Mol Biol. 1970 Aug;51(3):687–702. doi: 10.1016/0022-2836(70)90017-3. [DOI] [PubMed] [Google Scholar]
  9. Krupp G., Gross H. J. Rapid RNA sequencing: nucleases from Staphylococcus aureus and Neurospora crassa discriminate between uridine and cytidine. Nucleic Acids Res. 1979 Aug 10;6(11):3481–3490. doi: 10.1093/nar/6.11.3481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Maden B. E., Robertson J. S. Demonstration of the "5-8 S" ribosomal sequence in HeLa cell ribosomal precursor RNA. J Mol Biol. 1974 Aug 5;87(2):227–235. doi: 10.1016/0022-2836(74)90145-4. [DOI] [PubMed] [Google Scholar]
  11. Nazar R. N., Sitz T. O., Busch H. Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. J Biol Chem. 1975 Nov 25;250(22):8591–8597. [PubMed] [Google Scholar]
  12. Pace N. R., Walker T. A., Schroeder E. Structure of the 5.8S RNA component of the 5.8S-28S ribosomal RNA junction complex. Biochemistry. 1977 Nov 29;16(24):5321–5328. doi: 10.1021/bi00643a025. [DOI] [PubMed] [Google Scholar]
  13. Pavlakis G. N., Jordan B. R., Wurst R. M., Vournakis J. N. Sequence and secondary structure of Drosophila melanogaster 5.8S and 2S rRNAs and of the processing site between them. Nucleic Acids Res. 1979 Dec 20;7(8):2213–2238. doi: 10.1093/nar/7.8.2213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pene J. J., Knight E., Jr, Darnell J. E., Jr Characterization of a new low molecular weight RNA in HeLa cell ribosomes. J Mol Biol. 1968 May 14;33(3):609–623. doi: 10.1016/0022-2836(68)90309-4. [DOI] [PubMed] [Google Scholar]
  15. Schaak J., Mao J., Söll D. The 5.8S RNA gene sequence and the ribosomal repeat of Schizosaccharomyces pombe. Nucleic Acids Res. 1982 May 11;10(9):2851–2864. doi: 10.1093/nar/10.9.2851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schnare M. N., Gray M. W. Nucleotide sequence of an exceptionally long 5.8S ribosomal RNA from Crithidia fasciculata. Nucleic Acids Res. 1982 Mar 25;10(6):2085–2092. doi: 10.1093/nar/10.6.2085. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Silberklang M., Gillum A. M., RajBhandary U. L. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979;59:58–109. doi: 10.1016/0076-6879(79)59072-7. [DOI] [PubMed] [Google Scholar]
  18. Ursi D., Vandenberghe A., De Wachter R. The sequence of the 5.8 S ribosomal RNA of the crustacean Artemia salina. With a proposal for a general secondary structure model for 5.8 S ribosomal RNA. Nucleic Acids Res. 1982 Jun 11;10(11):3517–3530. doi: 10.1093/nar/10.11.3517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wengler G., Wengler G., Gross H. S. Replicative form of Semliki Forest virus RNA contains an unpaired guanosine. Nature. 1979 Dec 13;282(5740):754–756. doi: 10.1038/282754a0. [DOI] [PubMed] [Google Scholar]

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