Abstract
The nucleotide sequence of Drosophila melanogaster tRNA 5 Lys is pGCCCGGAUAm2GCUCAGDCGGDAGAGCA psi psi GGACUsU*UUt6A*A psi CCAAGGm7GDm5CCAGGGTm psi CAm1AGUCCCUGUUCGGGCGCCA. The sU* is probably 5-methylcarboxymethyl-2-thiouridine and t6A* is a mixture of modified derivatives of t6A including t6A itself and a component sensitive to treatment with cyanogen bromide. This tRNA 5 Lys is 95% homologous to the rabbit liver tRNA 5 Lys.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DeFranco D., Schmidt O., Söll D. Two control regions for eukaryotic tRNA gene transcription. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3365–3368. doi: 10.1073/pnas.77.6.3365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeFranco D., Sharp S., Söll D. Identification of regulatory sequences contained in the 5'-flanking region of Drosophila lysine tRNA2 genes. J Biol Chem. 1981 Dec 10;256(23):12424–12429. [PubMed] [Google Scholar]
- Dingermann T., Sharp S., Appel B., DeFranco D., Mount S., Heiermann R., Pongs O., Söll D. Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract. Nucleic Acids Res. 1981 Aug 25;9(16):3907–3918. doi: 10.1093/nar/9.16.3907. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunn R., Addison W. R., Gillam I. C., Tener G. M. The purification and properties of valine tRNAs of Drosophila melanogaster. Can J Biochem. 1978 Jun;56(6):618–623. doi: 10.1139/o78-093. [DOI] [PubMed] [Google Scholar]
- Dunn R., Delaney A. D., Gillam I. C., Hayashi S., Tener G. M., Grigliatti T., Misra V., Spurr M. G., Taylor D. M., Miller R. C., Jr Isolation and characterization of recombinant DNA plasmids carrying Drosophila tRNA genes. Gene. 1979 Nov;7(3-4):197–215. doi: 10.1016/0378-1119(79)90046-5. [DOI] [PubMed] [Google Scholar]
- England T. E., Uhlenbeck O. C. 3'-terminal labelling of RNA with T4 RNA ligase. Nature. 1978 Oct 12;275(5680):560–561. doi: 10.1038/275560a0. [DOI] [PubMed] [Google Scholar]
- Gergen J. P., Loewenberg J. Y., Wensink P. C. tRNA2Lys gene clusters in Drosophila. J Mol Biol. 1981 Apr 25;147(4):475–499. doi: 10.1016/0022-2836(81)90396-x. [DOI] [PubMed] [Google Scholar]
- Gupta R. C., Randerath K. Rapid print-readout technique for sequencing of RNA's containing modified nucleotides. Nucleic Acids Res. 1979 Aug 10;6(11):3443–3458. doi: 10.1093/nar/6.11.3443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayashi S., Gillam I. C., Delaney A. D., Dunn R., Tener G. M., Grigliatti T. A., Suzuki D. T. Hybridization of tRNAs of Drosophila melanogaster to polytene chromosomes. Chromosoma. 1980;76(1):65–84. doi: 10.1007/BF00292227. [DOI] [PubMed] [Google Scholar]
- Hovemann B., Sharp S., Yamada H., Söll D. Analysis of a drosophila tRNA gene cluster. Cell. 1980 Apr;19(4):889–895. doi: 10.1016/0092-8674(80)90080-x. [DOI] [PubMed] [Google Scholar]
- Jay E., Bambara R., Padmanabhan R., Wu R. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping. Nucleic Acids Res. 1974 Mar;1(3):331–353. doi: 10.1093/nar/1.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lillehaug J. R., Kleppe K. Phosphorylation of tRNA by T4 polynucleotide kinase. Nucleic Acids Res. 1977 Feb;4(2):373–380. doi: 10.1093/nar/4.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Raba M., Limburg K., Burghagen M., Katze J. R., Simsek M., Heckman J. E., Rajbhandary U. L., Gross H. J. Nucleotide sequence of three isoaccepting lysine tRNAs from rabbit liver and SV40-transformed mouse fibroblasts. Eur J Biochem. 1979 Jun;97(1):305–318. doi: 10.1111/j.1432-1033.1979.tb13115.x. [DOI] [PubMed] [Google Scholar]
- Randerath K., Gupta R. C., Randerath E. 3H and 32P derivative methods for base composition and sequence analysis of RNA. Methods Enzymol. 1980;65(1):638–680. doi: 10.1016/s0076-6879(80)65065-4. [DOI] [PubMed] [Google Scholar]
- Saneyoshi M., Nishimura S. Selective inactivation of amino acid acceptor and ribosome-binding activities of Escherichia coli tRNA by modification with cyanogen bromide. Biochim Biophys Acta. 1971 Aug 12;246(1):123–131. doi: 10.1016/0005-2787(71)90077-3. [DOI] [PubMed] [Google Scholar]
- Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
- Silberklang M., Gillum A. M., RajBhandary U. L. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs. Methods Enzymol. 1979;59:58–109. doi: 10.1016/0076-6879(79)59072-7. [DOI] [PubMed] [Google Scholar]
- Silverman S., Gillam I. C., Tener G. M., Söll D. The nucleotide sequence of lysine tRNA2 from Drosophila. Nucleic Acids Res. 1979 Feb;6(2):435–442. doi: 10.1093/nar/6.2.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
- Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
- Yen P. H., Sodja A., Cohen M., Jr, Conrad S. E., Wu M., Davidson N. Sequence arrangement of tRNA genes on a fragment of Drosophila melanogaster DNA cloned in E. coli. Cell. 1977 Aug;11(4):763–777. doi: 10.1016/0092-8674(77)90290-2. [DOI] [PubMed] [Google Scholar]


