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. 1982 Nov 11;10(21):6675–6694. doi: 10.1093/nar/10.21.6675

New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.

V A Efimov, S V Reverdatto, O G Chakhmakhcheva
PMCID: PMC326957  PMID: 7177853

Abstract

A rapid and convenient method for the synthesis of deoxyribooligonucleotides has been developed using the phosphotriester approach. The advantage of this methodology for work in solution was successfully demonstrated in synthesis of a number of DNA fragments up to 32-long. Adaptation of the presented method to solid-phase synthesis allows a pentadecamer to be assembled in 4-5 hours using dinucleotides as coupling units.

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Selected References

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