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. 1982 Nov 25;10(22):7387–7393. doi: 10.1093/nar/10.22.7387

Avian myeloblastosis reverse transcriptase deacylates tryptophanyl-tRNA.

L Sarih, M Fournier, F von der Haar, J Labouesse, S Litvak
PMCID: PMC327011  PMID: 6185923

Abstract

Tryptophanyl-tRNA can be used as a primer for RNA-dependent DNA synthesis by avian reverse transcriptase. We have determined that whereas the retroviral polymerase is not by itself capable of deacylating Trp-tRNATrp, the ester linkage between the 3' OH of the ribose moiety and the aminoacid can be very efficiently hydrolyzed when both the polymerase and the viral 35 S RNA are present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araya A., Hevia E., Litvak S. Study of the interactions between avian myeloblastosis virus reverse transcriptase and primer tRNA. Affinity labeling and inactivation of the enzyme by periodate-treated tRNATrp. Nucleic Acids Res. 1980 Sep 11;8(17):4009–4020. doi: 10.1093/nar/8.17.4009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Araya A., Keith G., Fournier M., Gandar J. C., Labouesse J., Litvak S. Photochemical cross-linking studies on the interactions of avian myeloblastosis virus reverse transcriptase with primer tRNATrp and TTP. Arch Biochem Biophys. 1980 Dec;205(2):437–448. doi: 10.1016/0003-9861(80)90127-7. [DOI] [PubMed] [Google Scholar]
  3. Araya A., Litvak S. Studies on the interaction of tRNA and avian myeloblastosis DNA polymerase. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):631–637. doi: 10.1101/sqb.1979.043.01.070. [DOI] [PubMed] [Google Scholar]
  4. Araya A., Sarih L., Litvak S. Reverse transcriptase mediated binding of primer tRNA to the viral genome. Nucleic Acids Res. 1979 Aug 24;6(12):3831–3843. doi: 10.1093/nar/6.12.3831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Baroudy B. M., Fournier M., Labouesse J., Papas T. S., Chirikjian J. G. tRNATrp (bovine) binding to the reverse transcriptase of avian myeloblastosis virus and function as a heterologous primer. Proc Natl Acad Sci U S A. 1977 May;74(5):1889–1893. doi: 10.1073/pnas.74.5.1889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bishop J. M. Retroviruses. Annu Rev Biochem. 1978;47:35–88. doi: 10.1146/annurev.bi.47.070178.000343. [DOI] [PubMed] [Google Scholar]
  7. Bonnet J. Studies on the mechanism of deacylation of aminoacyl-tRNAs by aminoacyl-tRNA synthetases in the absence of adenosine monophosphate and pyrophosphate. Biochimie. 1974;56(4):541–545. doi: 10.1016/s0300-9084(74)80071-4. [DOI] [PubMed] [Google Scholar]
  8. Cordell B., Stavnezer E., Friedrich R., Bishop J. M., Goodman H. M. Nucleotide sequence that binds primer for DNA synthesis to the avian sarcoma virus genome. J Virol. 1976 Aug;19(2):548–558. doi: 10.1128/jvi.19.2.548-558.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eiden J. J., Quade K., Nichols J. L. Interaction of tryptophan transfer RNA with Rous sarcoma virus 35S RNA. Nature. 1976 Jan 22;259(5540):245–247. doi: 10.1038/259245a0. [DOI] [PubMed] [Google Scholar]
  10. Fournier M., Labouesse J., Dirheimer G., Fix C., Keith G. Primary structure of bovine liver tRNATrp. Biochim Biophys Acta. 1978 Nov 21;521(1):198–208. doi: 10.1016/0005-2787(78)90262-9. [DOI] [PubMed] [Google Scholar]
  11. Harada F., Sawyer R. C., Dahlberg J. E. A primer ribonucleic acid for initiation of in vitro Rous sarcarcoma virus deoxyribonucleic acid synthesis. J Biol Chem. 1975 May 10;250(9):3487–3497. [PubMed] [Google Scholar]
  12. Houts G. E., Miyagi M., Ellis C., Beard D., Beard J. W. Reverse transcriptase from avian myeloblastosis virus. J Virol. 1979 Feb;29(2):517–522. doi: 10.1128/jvi.29.2.517-522.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Maelicke A., Sprinzl M., von der Haar F., Khwaja T. A., Cramer F. Structural studies on phenylalanine transfer ribonucleic acid from yeast with the spectroscopic label formycin. Eur J Biochem. 1974 Apr 16;43(3):617–625. doi: 10.1111/j.1432-1033.1974.tb03449.x. [DOI] [PubMed] [Google Scholar]
  14. Mérault G., Graves P. V., Labouesse B., Labouesse J. Influence of magnesium on the steady-state-derived order of substrate addition and product release in tRNATrp aminoacylation by beef pancreas tryptophan: tRNA ligase: significance of the deduced mechanism. Eur J Biochem. 1978 Jul 3;87(3):541–550. doi: 10.1111/j.1432-1033.1978.tb12405.x. [DOI] [PubMed] [Google Scholar]
  15. Ogilvie A., Huschka U., Kersten W. Control of protein synthesis in mammalian cells by aminoacylation of transfer ribonucleic acid. Biochim Biophys Acta. 1979 Dec 17;565(2):293–304. doi: 10.1016/0005-2787(79)90206-5. [DOI] [PubMed] [Google Scholar]
  16. Panet A., Haseltine W. A., Baltimore D., Peters G., Harada F., Dahlberg J. E. Specific binding of tryptophan transfer RNA to avian myeloblastosis virus RNA-dependent DNA polymerase (reverse transcriptase). Proc Natl Acad Sci U S A. 1975 Jul;72(7):2535–2539. doi: 10.1073/pnas.72.7.2535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Panet A., Weil G., Friis R. R. Binding of tryptophanyl-tRNA to the reverse transcriptase of replication-defective avian sarcoma viruses. J Virol. 1978 Nov;28(2):434–443. doi: 10.1128/jvi.28.2.434-443.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Peters G. G., Hu J. Reverse transcriptase as the major determinant for selective packaging of tRNA's into Avian sarcoma virus particles. J Virol. 1980 Dec;36(3):692–700. doi: 10.1128/jvi.36.3.692-700.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sprinzl M., Sternbach H., von der Haar F., Cramer F. Enzymatic incorporation of ATP and CTP analogues into the 3' end of tRNA. Eur J Biochem. 1977 Dec;81(3):579–589. doi: 10.1111/j.1432-1033.1977.tb11985.x. [DOI] [PubMed] [Google Scholar]
  20. Taylor J. M., Illmensee R. Site on the RNA of an avian sarcoma virus at which primer is bound. J Virol. 1975 Sep;16(3):553–558. doi: 10.1128/jvi.16.3.553-558.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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