Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Dec 11;10(23):7511–7520. doi: 10.1093/nar/10.23.7511

Nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC): the tRNAVal (UAC) gene contains a long intron.

H Deno, A Kato, K Shinozaki, M Sugiura
PMCID: PMC327026  PMID: 6296781

Abstract

The nucleotide sequences of tobacco chloroplast genes for elongator tRNAMet and tRNAVal (UAC) have been determined. The tRNAVal gene contains a 571 base pairs intron located in the anticodon loop. The tRNAVal gene is transcribed as a 750 bases precursor RNA molecule. Both tRNAs deduced from the DNA sequences show 97% sequence homologies with those of spinach chloroplasts.

Full text

PDF
7511

Images in this article

7516Image
on p.7516
7519Image
on p.7519

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  2. Calagan J. L., Pirtle R. M., Pirtle I. L., Kashdan M. A., Vreman H. J., Dudock B. S. Homology between chloroplast and prokaryotic initiator tRNA. Nucleotide sequence of spinach chloroplast methionine initiator tRNA. J Biol Chem. 1980 Oct 25;255(20):9981–9984. [PubMed] [Google Scholar]
  3. Driesel A. J., Crouse E. J., Gordon K., Bohnert H. J., Herrmann R. G., Steinmetz A., Mubumbila M., Keller M., Burkard G., Weil J. H. Fractionation and identification of spinach chloroplast transfer RNAs and mapping of their genes on the restriction map of chloroplast DNA. Gene. 1979 Aug;6(4):285–306. doi: 10.1016/0378-1119(79)90070-2. [DOI] [PubMed] [Google Scholar]
  4. Graf L., Kössel H., Stutz E. Sequencing of 16S--23S spacer in a ribosomal RNA operon of Euglena gracilis chloroplast DNA reveals two tRNA genes. Nature. 1980 Aug 28;286(5776):908–910. doi: 10.1038/286908a0. [DOI] [PubMed] [Google Scholar]
  5. Kashdan M. A., Dudock B. S. Structure of a spinach chloroplast threonine tRNA gene. J Biol Chem. 1982 Feb 10;257(3):1114–1116. [PubMed] [Google Scholar]
  6. Kato A., Shimada H., Kusuda M., Sugiura M. The nucleotide sequences of two tRNAAsn genes from tobacco chloroplasts. Nucleic Acids Res. 1981 Nov 11;9(21):5601–5607. doi: 10.1093/nar/9.21.5601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Koch W., Edwards K., Kössel H. Sequencing of the 16S-23S spacer in a ribosomal RNA operon of Zea mays chloroplast DNA reveals two split tRNA genes. Cell. 1981 Jul;25(1):203–213. doi: 10.1016/0092-8674(81)90245-2. [DOI] [PubMed] [Google Scholar]
  8. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orozco E. M., Jr, Hallick R. B. Euglena gracilis chloroplast transfer RNA transcription units. II. Nucleotide sequence analysis of a tRNAVal-tRNAAsn-tRNAArg-tRNALeu gene cluster. J Biol Chem. 1982 Mar 25;257(6):3265–3275. [PubMed] [Google Scholar]
  10. Orozco E. M., Jr, Rushlow K. E., Dodd J. R., Hallick R. B. Euglena gracilis chloroplast ribosomal RNA transcription units. II. Nucleotide sequence homology between the 16 S--23 S ribosomal RNA spacer and the 16 S ribosomal RNA leader regions. J Biol Chem. 1980 Nov 25;255(22):10997–11003. [PubMed] [Google Scholar]
  11. Pirtle R., Calagan J., Pirtle I., Kashdan M., Vreman H., Dudock B. The nucleotide sequence of spinach chloroplast methionine elongator tRNA. Nucleic Acids Res. 1981 Jan 10;9(1):183–188. doi: 10.1093/nar/9.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwarz Z., Jolly S. O., Steinmetz A. A., Bogorad L. Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3423–3427. doi: 10.1073/pnas.78.6.3423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwarz Z., Kössel H., Schwarz E., Bogorad L. A gene coding for tRNA is located near 5' terminus of 16S rRNA gene in Zea mays chloroplast genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4748–4752. doi: 10.1073/pnas.78.8.4748. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shinozaki K., Sugiura M. Sequence of the intercistronic region between the ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit and coupling factor beta subunit gene. Nucleic Acids Res. 1982 Aug 25;10(16):4923–4934. doi: 10.1093/nar/10.16.4923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sprinzl M., Gauss D. H. Compilation of tRNA sequences. Nucleic Acids Res. 1982 Jan 22;10(2):r1–55. [PMC free article] [PubMed] [Google Scholar]
  16. Sprouse H. M., Kashdan M., Otis L., Dudock B. Nucleotide sequence of a spinach chloroplast valine tRNA. Nucleic Acids Res. 1981 Jun 11;9(11):2543–2547. doi: 10.1093/nar/9.11.2543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Steinmetz A., Gubbins E. J., Bogorad L. The anticodon of the maize chloroplast gene for tRNA Leu UAA is split by a large intron. Nucleic Acids Res. 1982 May 25;10(10):3027–3037. doi: 10.1093/nar/10.10.3027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takaiwa F., Sugiura M. Nucleotide sequence of the 16S - 23S spacer region in an rRNA gene cluster from tobacco chloroplast DNA. Nucleic Acids Res. 1982 Apr 24;10(8):2665–2676. doi: 10.1093/nar/10.8.2665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Thomas P. S. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201–5205. doi: 10.1073/pnas.77.9.5201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tohdoh N., Shinozaki K., Sugiura M. Sequence of a putative promoter region for the rRNA genes of tobacco chloroplast DNA. Nucleic Acids Res. 1981 Oct 24;9(20):5399–5406. doi: 10.1093/nar/9.20.5399. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES