Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1982 Dec 11;10(23):7777–7789. doi: 10.1093/nar/10.23.7777

Nucleic acid binding properties of major proteins from the heterogeneous nuclear ribonucleoproteins of wheat.

Raziuddin, J O Thomas, W Szer
PMCID: PMC327045  PMID: 7155898

Abstract

Glycine-rich core hnRNP proteins purified from wheat bind tightly to single-stranded but not to double-stranded nucleic acids with a preference for natural RNA over single-stranded DNA. Binding results in i) a progressive disruption of the residual secondary structure of the polynucleotide and the formation of an extended nucleoprotein filament until a protein to polynucleotide weight ratio of about 5:1 is attained. As more protein is added, this is followed by ii) the formation of globular structures along the polynucleotide chain with a concomitant reduction in the contour length of the nucleoprotein complex. These two features of the interaction--unwinding and condensation into beads--are analogous to the previously described behavior of the major glycine-rich core hnRNP protein from Artemia salina (Thomas et al. (1981) Proc. Natl. Acad. Sci. USA 78, 2888) and may represent the basic functional properties of this relatively well conserved group of nuclear proteins.

Full text

PDF
7777

Images in this article

7781Image
on p.7781
7785Image
on p.7785

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beyer A. L., Christensen M. E., Walker B. W., LeStourgeon W. M. Identification and characterization of the packaging proteins of core 40S hnRNP particles. Cell. 1977 May;11(1):127–138. doi: 10.1016/0092-8674(77)90323-3. [DOI] [PubMed] [Google Scholar]
  3. Carmichael G. G. Isolation of bacterial and phage proteins by homopolymer RNA-cellulose chromatography. J Biol Chem. 1975 Aug 10;250(15):6160–6167. [PubMed] [Google Scholar]
  4. Christensen M. E., Beyer A. L., Walker B., Lestourgeon W. M. Identification of NG, NG-dimethylarginine in a nuclear protein from the lower eukaryote physarum polycephalum homologous to the major proteins of mammalian 40S ribonucleoprotein particles. Biochem Biophys Res Commun. 1977 Jan 24;74(2):621–629. doi: 10.1016/0006-291x(77)90348-5. [DOI] [PubMed] [Google Scholar]
  5. Christensen M. E., LeStourgeon W. M., Jamrich M., Howard G. C., Serunian L. A., Silver L. M., Elgin S. C. Distribution studies on polytene chromosomes using antibodies directed against hnRNP. J Cell Biol. 1981 Jul;90(1):18–24. doi: 10.1083/jcb.90.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fiers W., Contreras R., Duerinck F., Haegeman G., Iserentant D., Merregaert J., Min Jou W., Molemans F., Raeymaekers A., Van den Berghe A. Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene. Nature. 1976 Apr 8;260(5551):500–507. doi: 10.1038/260500a0. [DOI] [PubMed] [Google Scholar]
  7. Fuchs J. P., Judes C., Jacob M. Characterization of glycine-rich proteins from the ribonucleoproteins containing heterogeneous nuclear ribonucleic acid. Biochemistry. 1980 Mar 18;19(6):1087–1094. doi: 10.1021/bi00547a007. [DOI] [PubMed] [Google Scholar]
  8. Hamilton R. H., Künsch U., Temperli A. Simple rapid procedures for isolation of tobacco leaf nuclei. Anal Biochem. 1972 Sep;49(1):48–57. doi: 10.1016/0003-2697(72)90241-2. [DOI] [PubMed] [Google Scholar]
  9. Jones R. E., Okamura C. S., Martin T. E. Immunofluorescent localization of the proteins of nuclear ribonucleoprotein complexes. J Cell Biol. 1980 Jul;86(1):235–243. doi: 10.1083/jcb.86.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karn J., Vidali G., Boffa L. C., Allfrey V. G. Characterization of the non-histone nuclear proteins associated with rapidly labeled heterogeneous nuclear RNA. J Biol Chem. 1977 Oct 25;252(20):7307–7322. [PubMed] [Google Scholar]
  11. Keppler D. O., Kaiser W. Enzymatic analysis of guanine nucleotides in tissues and cells. Anal Biochem. 1978 May;86(1):147–153. doi: 10.1016/0003-2697(78)90328-7. [DOI] [PubMed] [Google Scholar]
  12. Marvil D. K., Nowak L., Szer W. A single-stranded nucleic acid-binding protein from Artemia salina. I. Purification and characterization. J Biol Chem. 1980 Jul 10;255(13):6466–6472. [PubMed] [Google Scholar]
  13. Mayrand S., Setyono B., Greenberg J. R., Pederson T. Structure of nuclear ribonucleoprotein: identification of proteins in contact with poly(A)+ heterogeneous nuclear RNA in living HeLa cells. J Cell Biol. 1981 Aug;90(2):380–384. doi: 10.1083/jcb.90.2.380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Northemann W., Seifert H., Heinrich P. C. The effect of sodium chloride on the structure of ribonucleoprotein particles from rat liver nuclei. Hoppe Seylers Z Physiol Chem. 1979 Jul;360(7):877–888. doi: 10.1515/bchm2.1979.360.2.877. [DOI] [PubMed] [Google Scholar]
  15. Nowak L., Marvil D. K., Thomas J. O., Boublik M., Szer W. A single-stranded nucleic acid-binding protein from Artemia salina. II. Interaction with nucleic acids. J Biol Chem. 1980 Jul 10;255(13):6473–6478. [PubMed] [Google Scholar]
  16. Riley M., Maling B. Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes. J Mol Biol. 1966 Sep;20(2):359–389. doi: 10.1016/0022-2836(66)90069-6. [DOI] [PubMed] [Google Scholar]
  17. Samarina O. P., Lukanidin E. M., Molnar J., Georgiev G. P. Structural organization of nuclear complexes containing DNA-like RNA. J Mol Biol. 1968 Apr 14;33(1):251–263. doi: 10.1016/0022-2836(68)90292-1. [DOI] [PubMed] [Google Scholar]
  18. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  19. Szer W. Ordered state of poly-uridylic acid above room temperature. J Mol Biol. 1966 Apr;16(2):585–587. doi: 10.1016/s0022-2836(66)80200-0. [DOI] [PubMed] [Google Scholar]
  20. Teng C. S., Teng C. T., Allfrey V. G. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription. J Biol Chem. 1971 Jun 10;246(11):3597–3609. [PubMed] [Google Scholar]
  21. Thomas J. O., Kolb A., Szer W. Structure of single-stranded nucleic acids in the presence of ribosomal protein S1. J Mol Biol. 1978 Aug 5;123(2):163–176. doi: 10.1016/0022-2836(78)90319-4. [DOI] [PubMed] [Google Scholar]
  22. Thomas J. O., Raziuddin, Sobota A., Boublik M., Szer W. A RNA helix-destabilizing protein is a major component of Artemia salina nuclear ribonucleoproteins. Proc Natl Acad Sci U S A. 1981 May;78(5):2888–2892. doi: 10.1073/pnas.78.5.2888. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES