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. 1982 Dec 20;10(24):7947–7957. doi: 10.1093/nar/10.24.7947

Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA.

P van Wezenbeek, P Vos, J van Boom, A van Kammen
PMCID: PMC327061  PMID: 6897677

Abstract

Double-stranded cDNA has been synthesized from RNA of a severe strain of potato spindle tuber viroid using a synthetic oligodeoxyribonucleotide as a primer. Upon cloning in bacteriophage M13mp9, two recombinant phages were selected to construct a pBR322-derived plasmid containing a complete viroid DNA copy. Elucidation of the nucleotide sequence revealed four differences with the previously established sequence of another PSTV strain, three of which were base exchanges and one a deletion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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