Abstract
A novel sequence-specific endonuclease has been isolated from Streptococcus cremoris F. ScrFI recognises the sequence: (formula; see text) and cleaves as indicated by the arrow ( ). It is the first enzyme to recognise this sequence and the first endonuclease reported from the lactic streptococci used in dairy fermentations.
Full text
PDF![8171](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/4be5b379b8db/nar00393-0275.png)
![8172](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/ec094e27e7c9/nar00393-0276.png)
![8173](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/18d2032c72a1/nar00393-0277.png)
![8174](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/e45dfc738006/nar00393-0278.png)
![8175](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/92b7d38d6e7f/nar00393-0279.png)
![8176](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/536b4f6ff370/nar00393-0280.png)
![8177](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/18ba8cd17839/nar00393-0281.png)
![8178](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/3e367e91b94a/nar00393-0282.png)
![8179](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c95f/327077/5bb3ad3acdff/nar00393-0283.png)
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Arber W. DNA modification and restriction. Prog Nucleic Acid Res Mol Biol. 1974;14(0):1–37. doi: 10.1016/s0079-6603(08)60204-4. [DOI] [PubMed] [Google Scholar]
- Bickle T. A., Pirrotta V., Imber R. Purification and properties of the Bg1I and II endonucleases. Methods Enzymol. 1980;65(1):132–138. doi: 10.1016/s0076-6879(80)65018-6. [DOI] [PubMed] [Google Scholar]
- Boussemaer J. P., Schrauwen P. P., Sourrouille J. L., Guy P. Multiple modification/restriction systems in lactic streptococci and their significance in defining a phage-typing system. J Dairy Res. 1980 Oct;47(3):401–409. doi: 10.1017/s0022029900021294. [DOI] [PubMed] [Google Scholar]
- Brownlee G. G., Sanger F. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969 Dec;11(2):395–399. doi: 10.1111/j.1432-1033.1969.tb00786.x. [DOI] [PubMed] [Google Scholar]
- Gelinas R. E., Myers P. A., Roberts R. J. Two sequence-specific endonucleases from Moraxella bovis. J Mol Biol. 1977 Jul;114(1):169–179. doi: 10.1016/0022-2836(77)90290-x. [DOI] [PubMed] [Google Scholar]
- Gingeras T. R., MIlazzo J. P., Roberts R. J. A computer assisted method for the determination of restriction enzyme recognifion sites. Nucleic Acids Res. 1978 Nov;5(11):4105–4127. doi: 10.1093/nar/5.11.4105. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Glynn I. M., Chappell J. B. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964 Jan;90(1):147–149. doi: 10.1042/bj0900147. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEHMAN I. R., NUSSBAUM A. L. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE). J Biol Chem. 1964 Aug;239:2628–2636. [PubMed] [Google Scholar]
- Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberts R. J. Restriction endonucleases. CRC Crit Rev Biochem. 1976 Nov;4(2):123–164. doi: 10.3109/10409237609105456. [DOI] [PubMed] [Google Scholar]
- Sanders M. E., Klaenhammer T. R. Restriction and modification in group N streptococci: effect of heat on development of modified lytic bacteriophage. Appl Environ Microbiol. 1980 Sep;40(3):500–506. doi: 10.1128/aem.40.3.500-506.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Donelson J. E., Coulson A. R., Kössel H., Fischer D. Use of DNA polymerase I primed by a synthetic oligonucleotide to determine a nucleotide sequence in phage fl DNA. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1209–1213. doi: 10.1073/pnas.70.4.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. doi: 10.1128/am.29.6.807-813.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wilson G. A., Young F. E. Purification and properties of the BamHI endonuclease. Methods Enzymol. 1980;65(1):147–153. doi: 10.1016/s0076-6879(80)65020-4. [DOI] [PubMed] [Google Scholar]
- Wu R., King C. T., Jay E. A new sequence-specific endonuclease from Streptococcus faecalis subsp. zymogenes. Gene. 1978 Dec;4(4):329–336. doi: 10.1016/0378-1119(78)90049-5. [DOI] [PubMed] [Google Scholar]