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. 1982 Dec 20;10(24):8211–8223. doi: 10.1093/nar/10.24.8211

On the cooperative and noncooperative binding of ethidium to DNA.

S A Winkle, L S Rosenberg, T R Krugh
PMCID: PMC327080  PMID: 7162990

Abstract

The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms obtained with calf thymus, C. perfringens, M. lysodeikticus, or poly(dG-dC) X (dG-dC) under the experimental conditions used (0.1 M NaCl).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer W., Vinograd J. Interaction of closed circular DNA with intercalative dyes. II. The free energy of superhelix formation in SV40 DNA. J Mol Biol. 1970 Feb 14;47(3):419–435. doi: 10.1016/0022-2836(70)90312-8. [DOI] [PubMed] [Google Scholar]
  2. Bontemps J., Fredericq E. Comparative binding study of the interaction of quinacrine and ethidium bromide with DNA and nucleohistone. Biophys Chem. 1974 Jun;2(1):1–22. doi: 10.1016/0301-4622(74)80019-0. [DOI] [PubMed] [Google Scholar]
  3. Bresloff J. L., Crothers D. M. Equilibrium studies of ethidium--polynucleotide interactions. Biochemistry. 1981 Jun 9;20(12):3547–3553. doi: 10.1021/bi00515a038. [DOI] [PubMed] [Google Scholar]
  4. Burns V. W. Fluorescence decay time characteristics of the complex between ethidium bromide and nucleic acids. Arch Biochem Biophys. 1969 Sep;133(2):420–424. doi: 10.1016/0003-9861(69)90471-8. [DOI] [PubMed] [Google Scholar]
  5. Chaires J. B., Dattagupta N., Crothers D. M. Studies on interaction of anthracycline antibiotics and deoxyribonucleic acid: equilibrium binding studies on interaction of daunomycin with deoxyribonucleic acid. Biochemistry. 1982 Aug 17;21(17):3933–3940. doi: 10.1021/bi00260a005. [DOI] [PubMed] [Google Scholar]
  6. Crothers D. M. Calculation of binding isotherms for heterogenous polymers. Biopolymers. 1968 Apr;6(4):575–584. doi: 10.1002/bip.1968.360060411. [DOI] [PubMed] [Google Scholar]
  7. Dattagupta N., Hogan M., Crothers D. M. Interaction of netropsin and distamycin with deoxyribonucleic acid: electric dichroism study. Biochemistry. 1980 Dec 23;19(26):5998–6005. doi: 10.1021/bi00567a009. [DOI] [PubMed] [Google Scholar]
  8. Davanloo P., Crothers D. M. Phase partition studies of actinomycin-nucleotide complexes. Biochemistry. 1976 Oct 5;15(20):4433–4438. doi: 10.1021/bi00665a015. [DOI] [PubMed] [Google Scholar]
  9. Erard M., Das G. C., de Murcia G., Mazen A., Pouyet J., Champagne M., Daune M. Ethidium bromide binding to core particle: comparison with native chromatin. Nucleic Acids Res. 1979 Jul 25;6(10):3231–3253. doi: 10.1093/nar/6.10.3231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hogan M., Dattagupta N., Crothers D. M. Transmission of allosteric effects in DNA. Nature. 1979 Apr 5;278(5704):521–524. doi: 10.1038/278521a0. [DOI] [PubMed] [Google Scholar]
  11. Houssier C., Hardy B., Fredericq E. Interaction of ethidium bromide with DNA. Optical and electrooptical study. Biopolymers. 1974 Jun;13(6):1141–1160. doi: 10.1002/bip.1974.360130607. [DOI] [PubMed] [Google Scholar]
  12. Hsieh T. S., Wang J. C. Thermodynamic properties of superhelical DNAs. Biochemistry. 1975 Feb 11;14(3):527–535. doi: 10.1021/bi00674a011. [DOI] [PubMed] [Google Scholar]
  13. Kastrup R. V., Young M. A., Krugh T. R. Ethidium bromide complexes with self-complementary deoxytetranucleotides. Demonstration and discussion of sequence preferences in the intercalative binding of ethidium bromide. Biochemistry. 1978 Nov 14;17(23):4855–4865. doi: 10.1021/bi00616a002. [DOI] [PubMed] [Google Scholar]
  14. Krugh T. R., Reinhardt C. G. Evidence for sequence preferences in the intercalative binding of ethidium bromide to dinucleoside monophosphates. J Mol Biol. 1975 Sep 15;97(2):133–162. doi: 10.1016/s0022-2836(75)80031-3. [DOI] [PubMed] [Google Scholar]
  15. Krugh T. R., Wittlin F. N., Cramer S. P. Ethidium bromide-dinucleotide complexes. Evidence for intercalation and sequence preferences in binding to double-stranded nucleic acids. Biopolymers. 1975 Jan;14(1):197–210. doi: 10.1002/bip.1975.360140114. [DOI] [PubMed] [Google Scholar]
  16. Krugh T. R., Young M. A. Daunorubicin and adriamycin facilitate actinomycin D binding to poly(dA-dT)-poly(dA-dT). Nature. 1977 Oct 13;269(5629):627–628. doi: 10.1038/269627a0. [DOI] [PubMed] [Google Scholar]
  17. LePecq J. B., Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967 Jul 14;27(1):87–106. doi: 10.1016/0022-2836(67)90353-1. [DOI] [PubMed] [Google Scholar]
  18. McGhee J. D., von Hippel P. H. Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice. J Mol Biol. 1974 Jun 25;86(2):469–489. doi: 10.1016/0022-2836(74)90031-x. [DOI] [PubMed] [Google Scholar]
  19. Müller W., Crothers D. M. Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands. Eur J Biochem. 1975 May;54(1):267–277. doi: 10.1111/j.1432-1033.1975.tb04137.x. [DOI] [PubMed] [Google Scholar]
  20. Müller W., Crothers D. M. Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands. Eur J Biochem. 1975 May;54(1):267–277. doi: 10.1111/j.1432-1033.1975.tb04137.x. [DOI] [PubMed] [Google Scholar]
  21. Patel D. J., Canuel L. L. Ethidium bromide-(dC-dG-dC-dG)2 complex in solution: intercalation and sequence specificity of drug binding at the tetranucleotide duplex level. Proc Natl Acad Sci U S A. 1976 Oct;73(10):3343–3347. doi: 10.1073/pnas.73.10.3343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pohl F. M., Jovin T. M., Baehr W., Holbrook J. J. Ethidium bromide as a cooperative effector of a DNA structure. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3805–3809. doi: 10.1073/pnas.69.12.3805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shanbhag V. P., Södergård R., Carstensen H., Albertsson P. A. A new method for the determination of the binding capacity of testosterone-estradiol-binding-globulin in human plasma. J Steroid Biochem. 1973 Sep;4(5):537–550. doi: 10.1016/0022-4731(73)90070-8. [DOI] [PubMed] [Google Scholar]
  24. Waring M. J. Complex formation between ethidium bromide and nucleic acids. J Mol Biol. 1965 Aug;13(1):269–282. doi: 10.1016/s0022-2836(65)80096-1. [DOI] [PubMed] [Google Scholar]
  25. Waring M. J., Wakelin L. P., Lee J. S. A solvent-partition method for measuring the binding of drugs to DNA. Application to the quinoxaline antibiotics echinomycin and triostin A. Biochim Biophys Acta. 1975 Oct 1;407(2):200–212. doi: 10.1016/0005-2787(75)90285-3. [DOI] [PubMed] [Google Scholar]
  26. Wells R. D., Goodman T. C., Hillen W., Horn G. T., Klein R. D., Larson J. E., Müller U. R., Neuendorf S. K., Panayotatos N., Stirdivant S. M. DNA structure and gene regulation. Prog Nucleic Acid Res Mol Biol. 1980;24:167–267. doi: 10.1016/s0079-6603(08)60674-1. [DOI] [PubMed] [Google Scholar]
  27. Winkle S. A., Krugh T. R. Equilibrium binding of carcinogens and antitumor antibiotics to DNA: site selectivity, cooperativity, allosterism. Nucleic Acids Res. 1981 Jul 10;9(13):3175–3186. doi: 10.1093/nar/9.13.3175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wu H. M., Dattagupta N., Hogan M., Crothers D. M. Unfolding of nucleosomes by ethidium binding. Biochemistry. 1980 Feb 19;19(4):626–634. doi: 10.1021/bi00545a004. [DOI] [PubMed] [Google Scholar]

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