Fig. 6.

CEH-63 and CEH-14 bound the mbr-1 promoter in Y1H screens and each other in Y2H assays. (A) One entire Y1H screen plate for the mbr-1 promoter bait yeast strain mated with the AD-TF array. Each AD-TF fusion is represented by 4 yeast colonies, each representing an independent assay. The assays for AD-CEH-63 and AD-CEH-14 are contained within the circle and show lacZ signals (blue) that are very clear, although weaker than for some other AD-TF fusions. (B) The circled region is enlarged for clarity. (C) The results are reproducible as demonstrated by this enlargement of a corresponding region from a repeat of the same Y1H screen. (D) One set of Y2H assays for CEH-63 and CEH-14. A set of six independent patch assays are presented for each of eight Y2H combinations (1–8). The DB-CEH-63 or DB-CEH-14 fusion was tested against the AD alone or the AD-CEH-14 from the TF array or the AD-CEH-63 from the TF array or the specifically constructed AD-CEH-63(whole). The AD-CEH-63 from the TF array lacks the N-terminus of the native CEH-63 while the AD-CEH-63(whole) includes the whole CEH-63 protein. Positive interactions are indicated by the blue colouration.