Fig. 2.

(A) Colony formation was performed using 22Rv1 and PC3 cells. For colony formation, cells were plated at ∼1000 cells per well and incubated for 48 h. After 48 h, media was replaced with fresh media containing α-mangostin and was repeated every 3–4 days until completion of the experiment. Statistical analysis performed using VassarStats software by one way analysis of variance and the Tukey test. ‘a’ versus control, ‘b’ versus 2.5 μM, ‘c’ versus 5 μM. (B) Phase contrast microscopic images (10x and 40x) of PrECs, PC3 and 22Rv1 cells with control and α-mangostin-treated cells (7.5 and 15 μM) are shown. Cells were treated for 48 h. (C) Cell cycle analysis was performed per manufacturer’s protocol using synchronized PC3 cells. Cells were synchronized for 36 h and then treated with α-mangostin for 24 h. These experiments were performed in triplicate and are represented by the mean along with standard deviation. These results were consistent with 22Rv1 cells treated with α-mangostin. Statistical analysis was performed using VassarStats software by one way analysis of variance and statistical significance was performed by the Tukey test with *P < 0.01 and **P < 0.001.