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. 1980 Jan 25;8(2):351–360. doi: 10.1093/nar/8.2.351

A method for detecting the coding DNA for a protein of known sequence in a collection of chimeric plasmids constructed with cDNAs.

G Roizes, E Pelaquier
PMCID: PMC327271  PMID: 6252545

Abstract

A rapid screening method for recognising plasmids containing copies of cDNA corresponding to proteins of known amino acid sequences, is described. The method is based on the computer prediction of the possible restriction sites in the cloned DNAs. It was tested on a series of proteins with known coding DNA sequences and a series of plasmid cloned cDNAs made from pure chicken globin mRNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baralle F. E., Brownlee G. G. AUG is the only recognisable signal sequence in the 5' non-coding regions of eukaryotic mRNA. Nature. 1978 Jul 6;274(5666):84–87. doi: 10.1038/274084a0. [DOI] [PubMed] [Google Scholar]
  2. Gordon J. I., Burns A. T., Christmann J. L., Deeley R. G. Cloning of a double-stranded cDNA that codes for a portion of chicken preproalbumin. A general method for isolating a specific DNA sequence from partially purified mRNA. J Biol Chem. 1978 Dec 10;253(23):8629–8639. [PubMed] [Google Scholar]
  3. Kedes L. H. Histone genes and histone messengers. Annu Rev Biochem. 1979;48:837–870. doi: 10.1146/annurev.bi.48.070179.004201. [DOI] [PubMed] [Google Scholar]
  4. Little P., Curtis P., Coutelle C., Van den Berg J., Dalgleish R., Malcolm S., Courtney M., Westaway D., Williamson R. Isolation and partial sequence of recombinant plasmids containing human alpha-, beta- and gamma-globin cDNA fragments. Nature. 1978 Jun 22;273(5664):640–643. doi: 10.1038/273640a0. [DOI] [PubMed] [Google Scholar]
  5. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  7. Paterson B. M., Roberts B. E., Kuff E. L. Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370–4374. doi: 10.1073/pnas.74.10.4370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Poon R., Kan Y. W., Boyer H. W. Sequence of the 3'-noncoding and adjacent coding regions of human gamma-globin mRNA. Nucleic Acids Res. 1978 Dec;5(12):4625–4630. [PMC free article] [PubMed] [Google Scholar]
  9. Roberts R. J. Restriction and modification enzymes and their recognition sequences. Gene. 1978 Nov;4(3):183–194. doi: 10.1016/0378-1119(78)90017-3. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Coulson A. R. The use of thin acrylamide gels for DNA sequencing. FEBS Lett. 1978 Mar 1;87(1):107–110. doi: 10.1016/0014-5793(78)80145-8. [DOI] [PubMed] [Google Scholar]
  11. Seeburg P. H., Shine J., Martial J. A., Baxter J. D., Goodman H. M. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone. Nature. 1977 Dec 8;270(5637):486–494. doi: 10.1038/270486a0. [DOI] [PubMed] [Google Scholar]
  12. Sures I., Lowry J., Kedes L. H. The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions. Cell. 1978 Nov;15(3):1033–1044. doi: 10.1016/0092-8674(78)90287-8. [DOI] [PubMed] [Google Scholar]

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