Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1980 Jan 25;8(2):361–375. doi: 10.1093/nar/8.2.361

Evidence implying DNA polymerase beta function in excision repair.

J A Siedlecki, J Szyszko, I Pietrzykowska, B Zmudzka
PMCID: PMC327272  PMID: 6252546

Abstract

Comparison was made of the ability of calf thymus DNA polymerases alpha and beta to replicate the following templates: native E. coli CR-34 DNA (T-DNA), calf thymus DNA activated by DNase I (act.DNA), BU-DNA (from E. coli CR-34 cells cultured on BUdR-containing medium) with damages resulting from incomplete excision repair, as well as thermally denatured act.DNA and BU-DNA (s.s.act.DNA and s.s.BU-DNA). 3H-TTP incorporation during extensive replication of act.DNA was similar for both enzymes, being, as expected, 40 times higher than for T-DNA. Likewise, the differences in the yield of the s.s.act.DNA or s.s.BU-DNA replication between both enzymes were negligible. In contrast, damaged native DNA was 6 - 30 times more extensively replicated by DNA polymerase beta than alpha. We propose that this is due to the greater ability of DNA polymerase beta compared with alpha to replicate single-stranded gaps, the presence of which is more likely in damaged BU-DNA than in T-DNA and act.DNA.

Full text

PDF
361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOLLUM F. J., SETLOW R. B. Ultraviolet inactivation of DNA primer activity. I. Effects of different wavelengths and doses. Biochim Biophys Acta. 1963 Apr 30;68:599–607. doi: 10.1016/0006-3002(63)90189-6. [DOI] [PubMed] [Google Scholar]
  2. Baril E., Mitchener J., Lee L., Baril B. Action of pancreatic DNase: requirements for activation of DNA as a template-primer for DNA polymerase. Nucleic Acids Res. 1977 Aug;4(8):2641–2653. doi: 10.1093/nar/4.8.2641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bollum F. J. Mammalian DNA polymerases. Prog Nucleic Acid Res Mol Biol. 1975;15(0):109–144. doi: 10.1016/s0079-6603(08)60118-x. [DOI] [PubMed] [Google Scholar]
  4. Bose K., Karran P., Strauss B. Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts. Proc Natl Acad Sci U S A. 1978 Feb;75(2):794–798. doi: 10.1073/pnas.75.2.794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cassani G. R., Bollum F. J. Oligodeoxythymidylate: polydeoxyadenylate and oligodeoxyadenylate: polydeoxythymidylate interactions. Biochemistry. 1969 Oct;8(10):3928–3936. doi: 10.1021/bi00838a008. [DOI] [PubMed] [Google Scholar]
  6. Chang L. M., Bollum F. J. A chemical model for transcriptional initiation of DNA replication. Biochem Biophys Res Commun. 1972 Feb 16;46(3):1354–1360. doi: 10.1016/s0006-291x(72)80124-4. [DOI] [PubMed] [Google Scholar]
  7. Chang L. M. Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. II. Initiation and fidelity of homopolymer replication. J Biol Chem. 1973 Oct 25;248(20):6983–6992. [PubMed] [Google Scholar]
  8. Chang L. M. Purification and properties of low molecular weight DNA polymerase from mammalian cells. Methods Enzymol. 1974;29:81–88. doi: 10.1016/0076-6879(74)29011-6. [DOI] [PubMed] [Google Scholar]
  9. Coetzee M. L., Chou R., Ove P. Selective response of DNA polymerase beta to bleomycin-induced breaks in DNA. Cancer Res. 1978 Nov;38(11 Pt 1):3621–3627. [PubMed] [Google Scholar]
  10. Fansler B. S., Loeb L. A. Sea urchin nuclear DNA polymerase. Methods Enzymol. 1974;29:53–70. doi: 10.1016/0076-6879(74)29009-8. [DOI] [PubMed] [Google Scholar]
  11. Herrick G., Delius H., Alberts B. Single-stranded DNA structure and DNA polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus. J Biol Chem. 1976 Apr 10;251(7):2142–2146. [PubMed] [Google Scholar]
  12. Krych M., Pietrzykowska I., Szyszko J., Shugar D. Genetic evidence for the nature, and excision repair, of DNA lesions resulting from incorporation of 5-bromouracil. Mol Gen Genet. 1979 Mar 20;171(2):135–143. doi: 10.1007/BF00269999. [DOI] [PubMed] [Google Scholar]
  13. Smith C. A., Hanawalt P. C. Phage T4 endonuclease V stimulates DNA repair replication in isolated nuclei from ultraviolet-irradiated human cells, including xeroderma pigmentosum fibroblasts. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2598–2602. doi: 10.1073/pnas.75.6.2598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spadari S., Weissbach A. RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha. Proc Natl Acad Sci U S A. 1975 Feb;72(2):503–507. doi: 10.1073/pnas.72.2.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Villani G., Boiteux S., Radman M. Mechanism of ultraviolet-induced mutagenesis: extent and fidelity of in vitro DNA synthesis on irradiated templates. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3037–3041. doi: 10.1073/pnas.75.7.3037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wang E., Henner D., Furth J. J. Duplication of single stranded DNA catalyzed by calf thymus DNA polymerase alpha. Nucleic Acids Res. 1976 Jan;3(1):129–147. doi: 10.1093/nar/3.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weissbach A. Eukaryotic DNA polymerases. Annu Rev Biochem. 1977;46:25–47. doi: 10.1146/annurev.bi.46.070177.000325. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES