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. 1980 Feb 11;8(3):425–440. doi: 10.1093/nar/8.3.425

Sequences hybridizing to mRNA, oligo(dT) and dsRNA from pre-mRNA are contiguous in the cloned mouse DNA fragments.

O N Tokarskaya, N A Tchurikov, P L Ivanov, D A Kramerov, A P Ryskov
PMCID: PMC327281  PMID: 6108553

Abstract

Fragments from the DNA of mouse embryos produced by restriction endonucleases HindIII were cloned in pBR322 plasmid and examined for the ability to hybridize in situ with [32P] labeled cDNA synthesized from the polysomal poly(A)+mRNA template. Several of the selected clones were examined for the presence of specific sequences inside the cloned mouse DNA fragments by the blotting procedure of southern [1]. The data obtained indicate that the majority of the cloned mouse DNA fragments contained sequences hybridizing with cDNA, oligo(dT) and double-stranded regions from pre-mRNA. The results of hybridization experiments and double digestion with HindIII+HaeIII endonucleases provide evidence that these sequences could be contiguous in the given restriction DNA fragments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  2. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  3. Finnegan D. J., Rubin G. M., Young M. W., Hogness D. S. Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1053–1063. doi: 10.1101/sqb.1978.042.01.106. [DOI] [PubMed] [Google Scholar]
  4. Flavell R. A., Van den Berg F. M., Grosveld G. C. Isolation and characterization of the oligo(dA-dT) clusters and their flanking DNA segments in the rabbit genome. J Mol Biol. 1977 Oct 5;115(4):715–735. doi: 10.1016/0022-2836(77)90111-5. [DOI] [PubMed] [Google Scholar]
  5. Georgiev G. P., Ryskov A. P., Coutelle C., Mantieva V. L., Avakyan E. R. On the structure of transcriptional unit in mammalian cells. Biochim Biophys Acta. 1972 Jan 31;259(2):259–283. doi: 10.1016/0005-2787(72)90066-4. [DOI] [PubMed] [Google Scholar]
  6. Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
  7. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ilyin Y. V., Tchurikov N. A., Ananiev E. V., Ryskov A. P., Yenikolopov G. N., Limborska S. A., Maleeva N. E., Gvozdev V. A., Georgiev G. P. Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):959–969. doi: 10.1101/sqb.1978.042.01.097. [DOI] [PubMed] [Google Scholar]
  9. Jelinek W. R. Inverted repeated DNA from Chinese hamster ovary cells studied with cloned DNA fragments. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2679–2683. doi: 10.1073/pnas.75.6.2679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jelinek W., Darnell J. E. Double-stranded regions in heterogeneous nuclear RNA from Hela cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2537–2541. doi: 10.1073/pnas.69.9.2537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kramerov D. A., Grigoryan A. A., Ryskov A. P., Georgiev G. P. Long double-stranded sequences (dsRNA-B) of nuclear pre-mRNA consist of a few highly abundant classes of sequences: evidence from DNA cloning experiments. Nucleic Acids Res. 1979 Feb;6(2):697–713. doi: 10.1093/nar/6.2.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kramerov D. A., Ryskov A. P., Georgiev G. P. The structural organization of nuclear pre-mRNA. II. Very long double-stranded structures in nuclear pre-mRNA. Biochim Biophys Acta. 1977 Apr 4;475(3):461–475. doi: 10.1016/0005-2787(77)90062-4. [DOI] [PubMed] [Google Scholar]
  13. Prensky W., Steffensen D. M., Hughes W. L. The use of iodinated RNA for gene localization. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1860–1864. doi: 10.1073/pnas.70.6.1860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ryskov A. P., Farashyan V. R., Georgiev G. P. Ribonuclease-stable base sequences specific exclusively for giant dRNA. Biochim Biophys Acta. 1972 Apr 12;262(4):568–572. doi: 10.1016/0005-2787(72)90502-3. [DOI] [PubMed] [Google Scholar]
  15. Ryskov A. P., Kramerov D. A., Georgiev G. P. The structural organization of nuclear messenger RNA precursor. I. Reassociation and hybridization properties of double-stranded hairpin-like loops in messenger RNA precursor. Biochim Biophys Acta. 1976 Oct 4;447(2):214–229. doi: 10.1016/0005-2787(76)90344-0. [DOI] [PubMed] [Google Scholar]
  16. Ryskov A. P., Saunders G. F., Farashyan V. R., Georgiev G. P. Double-helical regions in nuclear precursor of mRNA (pre-mRNA). Biochim Biophys Acta. 1973 Jun 8;312(1):152–164. doi: 10.1016/0005-2787(73)90060-9. [DOI] [PubMed] [Google Scholar]
  17. Ryskov A. P., Tokarskaya O. V., Georgiev G. P., Coutelle C., Thiele B. Globin mRNA contains a sequence complementary to double-stranded region of nuclear pre-mRNA. Nucleic Acids Res. 1976 Jun;3(6):1487–1498. doi: 10.1093/nar/3.6.1487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ryskov A. P., Yenikolopov G. N., Limborska S. A. Complementary regions of the nuclear precursor of messenger RNA. FEBS Lett. 1974 Oct 1;47(1):98–102. doi: 10.1016/0014-5793(74)80434-5. [DOI] [PubMed] [Google Scholar]
  19. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  20. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  21. Wienand U., Schwarz Z., Feix G. Electrophoretic elution of nucleic acids from gels adapted for subsequent biological tests. Application for analysis of mRNAs from maize endosperm. FEBS Lett. 1979 Feb 15;98(2):319–323. doi: 10.1016/0014-5793(79)80208-2. [DOI] [PubMed] [Google Scholar]

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