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. 1980 Feb 11;8(3):601–610. doi: 10.1093/nar/8.3.601

Elongation of oligonucleotides in the 3'-direction with activated mononucleotides and their analogs using RNA ligase.

E Ohtsuka, T Miyake, K Nagao, H Uemura, S Nishikawa, M Sugiura, M Ikehara
PMCID: PMC327293  PMID: 7443536

Abstract

P1-Adenosine 5'-P2-2',3'-ethoxymethylidene nucleosides [A(5')ppN(Em)] from four common nucleosides have been prepared and used for single addition of nucleotides to elongate oligonucleotide chains in the 3'-direction in RNA ligase reaction. U-U-C, T-U-C and A-C-C were used as acceptors. Structural dependence in these acceptors was found to be smaller compared to joining reactions between oligonucleotides. Adenosine analogs including 8-bromo-, 2'-fluoro-, 2'-azido-, 8,2'-O-cyclo-, 8,2'-S-cyclo-adenosine, arabinosyladenine and 2'-deoxyadenosine were added to the 3'-end of A-C-C by adenylation chemically followed by joining with RNA ligase. Symmetrical 5'-pyrophosphates of 8-bromo-, 2'-fluoro- and 2'-azido-adenosine were not recognized as donor substrates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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