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. 1981 Jul 10;9(13):3077–3088. doi: 10.1093/nar/9.13.3077

Yeast ochre suppressor SUQ5-ol is an altered tRNA Ser UCA.

C Waldron, B S Cox, N Wills, R F Gesteland, P W Piper, D Colby, C Guthrie
PMCID: PMC327332  PMID: 7024909

Abstract

Ochre suppressor tRNA was partially purified from strains of Saccharomyces cerevisiae containing the serine-inserting class III suppressor SUQ5-ol. RNA sequence analysis of this tRNA indicated that the suppressor is derived from a UCA-decoding tRNA Ser by a G leads to U substitution in the middle position of the anticodon. The suppressor further differs from the wild-type UCA-decoding tRNA Ser in that the mutant anticodon lacks the modified uridine found in the wobble position of the wild-type tRNA and contains instead another modification in or near the anticodon.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins J. F., Lewis J. B., Anderson C. W., Gesteland R. F. Enhanced differential synthesis of proteins in a mammalian cell-free system by addition of polyamines. J Biol Chem. 1975 Jul 25;250(14):5688–5695. [PubMed] [Google Scholar]
  2. Capecchi M. R., Hughes S. H., Wahl G. M. Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. Cell. 1975 Nov;6(3):269–277. doi: 10.1016/0092-8674(75)90178-6. [DOI] [PubMed] [Google Scholar]
  3. Etcheverry T., Colby D., Guthrie C. A precursor to a minor species of yeast tRNASer contains an intervening sequence. Cell. 1979 Sep;18(1):11–26. doi: 10.1016/0092-8674(79)90349-0. [DOI] [PubMed] [Google Scholar]
  4. Fradin A., Gruhl H., Feldmann H. Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels. FEBS Lett. 1975 Feb 1;50(2):185–189. doi: 10.1016/0014-5793(75)80485-6. [DOI] [PubMed] [Google Scholar]
  5. Gesteland R. F., Wolfner M., Grisafi P., Fink G., Botstein D., Roth J. R. Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA. Cell. 1976 Mar;7(3):381–390. doi: 10.1016/0092-8674(76)90167-7. [DOI] [PubMed] [Google Scholar]
  6. Goodman H. M., Olson M. V., Hall B. D. Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453–5457. doi: 10.1073/pnas.74.12.5453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmes W. M., Hurd R. E., Reid B. R., Rimerman R. A., Hatfield G. W. Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1068–1071. doi: 10.1073/pnas.72.3.1068. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kohli J., Kwong T., Altruda F., Söll D., Wahl G. Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. J Biol Chem. 1979 Mar 10;254(5):1546–1551. [PubMed] [Google Scholar]
  9. Liebman S. W., Stewart J. W., Sherman F. Serine substitutions caused by an ochre suppressor in yeast. J Mol Biol. 1975 Jun 5;94(4):595–610. doi: 10.1016/0022-2836(75)90324-1. [DOI] [PubMed] [Google Scholar]
  10. Ono B. I., Stewart J. W., Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. doi: 10.1016/0022-2836(79)90309-7. [DOI] [PubMed] [Google Scholar]
  11. Pearson R. L., Weiss J. F., Kelmers A. D. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns. Biochim Biophys Acta. 1971 Feb 11;228(3):770–774. doi: 10.1016/0005-2787(71)90748-9. [DOI] [PubMed] [Google Scholar]
  12. Piper P. W. A correlation between a recessive lethal amber suppressor mutation in Saccharomyces cerevisiae and an anticodon change in a minor serine transfer RNA. J Mol Biol. 1978 Jun 25;122(2):217–235. doi: 10.1016/0022-2836(78)90037-2. [DOI] [PubMed] [Google Scholar]
  13. Piper P. W., Wasserstein M., Engbaek F., Kaltoft K., Celis J. E., Zeuthen J., Liebman S., Sherman F. Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon. Nature. 1976 Aug 26;262(5571):757–761. doi: 10.1038/262757a0. [DOI] [PubMed] [Google Scholar]
  14. Rafalski A., Kohli J., Agris P., Söll D. The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucleic Acids Res. 1979 Jun 25;6(8):2683–2695. doi: 10.1093/nar/6.8.2683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rubin G. M. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed] [Google Scholar]
  16. Saneyoshi M., Oashi Z., Harada F., Nishimura S. Isolation and characterization of 2-methyladenosine from Escherichia coli tRNA Glu 2 , tRNA Asp 1 , tRNA His 1 and tRNA Arg . Biochim Biophys Acta. 1972 Feb 23;262(1):1–10. [PubMed] [Google Scholar]
  17. Wetzel R., Kohli J., Altruda F., Söll D. Identification and nucleotide sequence of the sup8-e UGA-suppressor leucine tRNA from Schizosaccharomyces pombe. Mol Gen Genet. 1979 May 4;172(2):221–228. doi: 10.1007/BF00268286. [DOI] [PubMed] [Google Scholar]

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