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. 1981 Aug 11;9(15):3779–3791. doi: 10.1093/nar/9.15.3779

Nonhistone chromatin proteins HMG-14 and HMG-17 bind preferentially to single-stranded DNA.

P J Isackson, G R Reeck
PMCID: PMC327391  PMID: 7279673

Abstract

Proteins extracted from chicken erythrocyte chromatin with 0.35 M NaCl were subjected to sequential chromatography on columns containing immobilized double-stranded and single-stranded DNA's. Two-dimensional electrophoresis of protein fractions revealed that HMG-14 and HMG-17 are among the proteins that are retained by the single-stranded DNA column in 0.2 M NaCl/l mM Tris-Cl (pH 7.5) after having failed to be retained by the double-stranded column under the same conditions. That suggests that those two proteins possess preferential affinity for single-stranded DNA. Further evidence for that was provided by chromatography of purified HMG-14 and of purified HMG-17 on single-stranded and double-stranded DNA columns. We discuss the possible relevance of our results to suggested functions of HMG-14 and HMG-17.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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