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. 1981 Aug 11;9(15):3793–3807. doi: 10.1093/nar/9.15.3793

Replication of SV40 chromatin in extracts from eggs of Xenopus laevis.

A Richter, B Otto, R Knippers
PMCID: PMC327392  PMID: 6269093

Abstract

Simian virus 40 (SV40) nucleoprotein complexes were prepared from lytically infected cells and used as primer-templates for DNA replication in protein extracts from Xenopus eggs. We found that nucleoprotein containing replicating SV40 DNA served as primer-template while nucleoprotein with nonreplicating SV40 DNA was ineffective. In vitro DNA synthesis begins with short DNA fragments ("Okazaki fragments") which are, in later steps, joined to give unit length SV40 DNA strands, suggesting that in vivo initiated rounds of replication are completed in vitro in the Xenopus system. This conclusion is supported by a restriction enzyme analysis showing that in vitro DNA synthesis occurs in fragments distal to the SV40 origin of replication. Our studies indicate that SV40 DNA replication in Xenopus extracts can be used an an experimental system to study the biochemistry of replicative DNA chain elongation in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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