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. Author manuscript; available in PMC: 2012 Apr 13.
Published in final edited form as: Nature. 2011 Oct 12;478(7368):E1–E3. doi: 10.1038/nature10493

Figure 1. InsP3R channels are clustered before exposure to InsP3, with gating properties unaltered by clustering.

Figure 1

a, NA in nuclear membrane patches with no pre-exposure to InsP3 obtained from InsP3R3 expressing DT40-KO cells. Note nonlinear square-root scale for frequency axis. b, Po observed under saturating [InsP3] and sub-optimal [Ca2+]i in multi- and apparent single-channel current records for recombinant rat InsP3R3 (r-3) channels expressed in DT40-KO (DK) cells or Xenopus oocytes (Xo), endogenous Xenopus InsP3R1 (X-1) channels from Xenopus oocytes (Xo), and endogenous insect InsP3R (iR) channels from Sf9 cells. Concentrations of free ATP4− ([ATP]f) in the pipette solutions used are indicated. Mean Po with s.e.m. (as error bars) and Po for individual current records are shown, together with mean Po from Rahman et al.1 c, Po of r-3 channels in DK cells in optimal [Ca2+]i and sub-saturating [InsP3], ligand conditions not investigated in Rahman et al.1. Same symbols as in b are used.