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. 1981 Aug 11;9(15):3863–3872. doi: 10.1093/nar/9.15.3863

Characterization of radiation damage to DNA by reaction with borohydride.

K A Schellenberg, J Shaeffer, R K Nichols, D Gates
PMCID: PMC327397  PMID: 7279674

Abstract

Irradiation of aqueous solutions of native calf thymus DNA with x-rays produced functional groups that reacted with sodium borohydride. The DNA was labeled with tritium from NaB3H4 to the extent of 2.0 x 10(-10) atom/dalton/rad. The presence of cysteamine or other radical scavengers, or saturation of the solution with nitrogen during irradiation decreased the labeling. After mild acid hydrolysis, the major tritium-containing moiety was identical with 2,3-dihydroxy-2-methylpropanoic acid in all chromatographic systems tested. The suggested mechanism of labeling involved reduction by borohydride of the potential aldehyde at carbon 6 of thymine glycol residues present in the irradiated DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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