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. 1981 Aug 25;9(16):3907–3918. doi: 10.1093/nar/9.16.3907

Transcription of cloned tRNA and 5S RNA genes in a Drosophila cell free extract

Theodor Dingermann 1, Stephen Sharp 1, Bernd Appel 1, Donald DeFranco 1, Stephen Mount 1, Reinhard Heiermann *, Olaf Pongs *, Dieter Söll 1
PMCID: PMC327404  PMID: 6170932

Abstract

We describe the preparation of a cell-free extract from Drosophila Kc cells which allows transcription of a variety of cloned eukaryotic RNA polymerase III genes. The extract has low RNA-processing nuclease activity and thus the major products obtained are primary transcripts.

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Selected References

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